Timothy Lohman

Timothy Lohman

Timothy M. Lohman, Ph.D.

Brennecke Prof of Biophysics
Biochemistry and Molecular Biophysics

Lab Website
Research Opportunities
Publications

 
 
 

Office: 2801 North Building
Phone: 314-362-4393
Email: lohman at biochem.wustl.edu
 

Research

The Lohman laboratory focuses on obtaining a molecular understanding of the mechanisms of protein-nucleic acid interactions involved in DNA metabolism, in particular, DNA motor proteins (helicases/translocases) and single stranded DNA binding proteins. Thermodynamic, kinetic, structural and single molecule approaches are used to probe these interactions at the molecular level.

Lohman lab research

Most Recent Publications

  • Suksombat, S., Khafizov, R., Kozlov, A.G., Lohman, T.M. and Chemla, Y. Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways. e-Life doi.org/10.7554/eLife.08193 (2015). [Abstract]
  • Comstock, M.J., Whitely, K.D., Jia, H., Sokoloski, J., Lohman, T., Ha, T. and Chemla, Y. Protein structure. Direct observations of structure-function relationship in a nucleic acid processing enzyme. Science 348:352-354 (2015). [Abstract]
  • Petrova, V., Chen, S., Molzberger, E., Tomko, E., Chitteni-Pattu, S., Jia, H., Ordabayev, Y., Lohman, T. and Cox, M. Active displacement of RecA filaments by UvrD translocase activity. Nucleic Acids Res. 43:4133-4139 (2015). [Abstract]
  • Kozlov, A.G., Weiland, E., Mittal, A., Waldman, V., Antony, E., Fazio, N., Pappu, R.V. and Lohman, T.M. Intrinsically disordered C-terminal tails of E. coli single stranded DNA binding protein regulated cooperative binding to single stranded DNA. J Mol Biol 427:763-774 (2015). [Abstract]
  • Nguyen, B., Sokoloski, J.E., Galletto, R., Elson, E.L., Wold, M.S. and Lohman, T.M. Diffusion of human replication protein A along single stranded DNA. J Mol Biol 426:3246-3261 (2014) [Abstract]
  • Lee, K.S., Marciel, A.B., Kozlov, A.G., Schroeder, C.M., Lohman, T.M. and Ha, T. Ultrafast redistribution of E. coli SSB along long single-stranded DNA via intersegment transfer. J. Mol. Biol. 426:2413-2421 (2014). [Abstract]
  • Antony, E., Weiland, E., Yuan, Q., Manhart, C. M., Nguyen, B., Kozlov, A. G., McHenry, C. S. and Lohman, T. M. Multiple C-terminal tails within a single E. coli SSB homotetramer coordinate DNA replication and repair. J. Mol. Biol. 425:4802-4819 (2013). [Abstract]
  • Qiu, Y., Antony, E., Doganay, S., Koh, H., Lohman, T. M. and Myong, S. Srs2 prevents Rad51 filament formation by repetitive motion on DNA. Nature Communications 4:2281 (2013). [Abstract]
  • Lee, K.S., Balci, H., Jia, H., Lohman, T.M. and Ha, T. Direct imaging of single UvrD helicase dynamics on long single-stranded DNA. Nature Communications 4:1878 (2013). [Abstract]
  • Xie, F., Wu, C.G., Weiland, E. and Lohman, T.M. Asymmetric regulation of bipolar single-stranded DNA translocation by the two morors within E. coli RecBCD helicase. J Biol Chem 288:1055-1064 (2013). [Abstract]
  • Kozlov, A.G., Galletto, R. and Lohman, T.M. SSB-DNA binding monitored by fluorescence intensity and anisotropy. Methods Mol Biol 922:55-83 (2012). [Abstract]
  • Kozlov, A.G. and Lohman, T.M. SSB binding to ssDNA using isothermal titration calorimetry. Methods Mol Biol 922:37-54 (2012). [Abstract]
  • Wu, C.G., Xie, F. and Lohman, T.M. The primary and secondary translocase activities within E. coli RecBC helicase are tightly coupled to ATP hydrolysis by the RecB motor. J Mol Biol 423:303-314. (2012). [Abstract]
  • Ha, T., Kozlov, A.G. and Lohman, T.M. Single molecule views of protein movement on single-stranded DNA. Annual Review of Biophysics 45:295-319 (2012). [Abstract]
  • Fischer, C.J., Tomko, E.J., Wu, C.G. and Lohman, T.M. Fluorescence methods to study DNA translocation and unwinding kinetics by nucleic acid motors. Methods Mol Biol 875:85-104 (2012). [Abstract]
  • Antony, E., Korolev, S., Weiland, E.A. and Lohman, T.M. Plasmodium falciparum SSB tetramer wraps single stranded DNA with similar topology but opposite polarity to E. coli SSB. J Mol Biol 420:269-283 (2012). [Abstract]
  • Antony, E., Kozlov, A.G., Nguyen, B. and Lohman, T.M. Plasmodium falciparum SSB tetramer binds single stranded DNA only in a fully wrapped mode. J Mol Biol 420:284-295 (2012). [Abstract]
  • Tomko, E.J., Fischer, C.J. and Lohman, T.M. Single stranded DNA translocation of E. coli UvrD monomer is tightly coupled to ATP hydrolysis. J Mol Biol 418:32-46 (2012). [Abstract]
  • Balci, H., Arslan, S., Myong, S., Lohman, T.M. and Ha, T. Single-molecule nanopositioning: Structural transitions of a helicase-DNA complex during ATP hydrolysis. Biophys J 101:976-984 (2011). [Abstract]
  • Niedziela-Majka, A., Maluf, N.K., Antony, E., Lohman, T.M. and Ha, T. Self-assembly of E. coli MutL and its complexes with DNA. Biochemistry 50:7868-7880 (2011). [Abstract]
  • Zhou, R., Kozlov, A.G., Roy, R., Zhang, J., Korolev, S., Lohman, T.M. and Ha, T. SSB functions as a sliding platform that migrates on DNA via reptation. Cell 146:222-232 (2011). [Abstract]
  • Jia, H., Korolev, S., Niedziela-Majka, A., Maluf, N.K., Gauss, G.H., Myong, S., Ha, T., Waksman, G. and Lohman, T.M. Rotations of the 2B sub-domain of E. coli UvrD helicase/translocase coupled to nucleotide and DNA binding. J Mol Biol 411:633-648 (2011). [Abstract]
  • Kozlov, A.G. and Lohman, T.M. E. coli SSB tetramer binds the first and second molecules of (dT)(35) with heat capacities of opposite sign. Biophys Chem 159:48-57 (2011). [Abstract]
  • Tomko, E.J., Jia, H., Park, J., Maluf, N.K., Ha, T. and Lohman, T.M. 5′-Single-stranded/duplex DNA junctions are loading sites for E. coli UvrD translocase. EMBO J 29:3826-3839 (2010). [Abstract]
  • Antony, E. and Lohman, T.M. “Non-hexameric SF1 DNA helicases and translocases”. Encyclopedia of Biological Chemistry, (2nd edition) W.J. Lennarz and M.D. Lane, eds. Elsevier Science (2010).
  • Wu, C.G., Bradford, C. and Lohman, T.M. Escherichia coli RecBC helicase has two translocase activities controlled by a single ATPase motor. Nat Struct Mol Biol 17:1210-1217 (2010). [Abstract]
  • Kozlov, A.G., Eggington, J.M., Cox, M.M. and Lohman, T.M. Binding of the Dimeric Deinococcus radiodurans SSB protein to single-stranded DNA. Biochemistry 49:8266-8275 (2010). [Abstract]
  • Park, J., Myong, S., Niedziela-Majka, A., Lee, K.S., Yu, J., Lohman, T.M. and Ha, Taekjip, T. PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps. Cell 142:544-555 (2010). [Abstract]
  • Lohman, T.M. Clipping along. J Mol Biol 399:663-664 (2010). [Abstract]
  • Tomko, E.J., Fischer, C.J. and Lohman, T.M. Ensemble methods for monitoring enzyme translocation along single stranded nucleic acids. Methods 51:269-276 (2010). [Abstract]

  • Kozlov, A.G., Cox, M.M. and Lohman, T.M. Regulation of single stranded DNA binding by the C-termini of E. coli SSB protein. J Biol Chem 285:17546-17252 (2010). [Abstract]

  • Kozlov, A.G., Jezewska, M.J., Bujalowski, W. and Lohman, T.M. Binding specificity of E. coli SSB protein for the chi subunit of DNA pol III holoenzyme and PriA helicase. Biochemistry 49:3555-3566 (2010). [Abstract]
  • Fischer, C.J., Wooten, L., Tomko, E.J. and Lohman, T.M. Kinetics of motor protein translocation on single-stranded DNA. Methods Mol Biol 587:45-56 (2010). [Abstract]

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