The Greenberg lab focuses on the generation and transduction of forces by molecular motors, with an emphasis on human disease. The lab uses an array of biochemical, biophysical, and cell biological techniques to probe the function and regulation of these motors over a range of scales that extends from single molecules to tissues. Currently, the lab is studying the molecular basis of heart failure.
Greenberg M.J., Shuman H., and Ostap E.M.
Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers.
Methods Mol Biol. 1486:483-509. (2017)
Kee, A.J., Yang, L., Lucas, C.A., Greenberg, M.J., Martel, N., Leong, G.M., Hughes, W.E., Cooney, G.J., James, D.E., Ostap, E.M., Han, W., Gunning, P.W. and Hardeman, E.C.
An actin filament population defined by the tropomyosin Tpm3.1 regulates glucose uptake.
Traffic (Copenhagen, Denmark). 16:691-711 (2015). (Abstract)
Greenberg, M.J., Lin, T., Shuman, H. and Ostap, E.M.
Mechanochemical tuning of myosin-I by the N-terminal region.
PNAS 112:E3337-E3344 (2015). (Abstract)
Greenberg, M.J., Shuman, H. and Ostap, E.M.
Inherent force-dependent properties of β-cardiac myosin contribute to the force-velocity relationship of cardiac muscle.
Biophysical J 107:L41-4 (2014). (Abstract)
Shuman, H., Greenberg, M.J., Zwolak, A., Lin, T., Sindelar, C.V., Dominguez, R. and Ostap, E.M.
A vertebrate myosin-I structure reveals unique insights into myosin mechanochemical tuning.
PNAS 111:2116-2121 (2014). (Abstract)
Greenberg, M.J. and Ostap, E.M.
Regulation and control of myosin-I by the motor and light chain-binding domains.
Trends in Cell Biology. 23:81-9 (2013). (Abstract)
Greenberg, M.J., Lin, T., Goldman, Y.E., Shuman, H. and Ostap, E.M.
Myosin IC generates power over a range of loads via a new tension-sensing mechanism.
Proc Natl Acad Sci U S A. 109:E2433-E2440 (2012). (Abstract)
Lewis, J.H., Greenberg, M.J., Laakso, J.M., Shuman, H. and Ostap, E.M.
Calcium regulation of myosin-I tension sensing.
Biophysical J. 102:2799-2807 (2012). (Abstract)