John
Jean measures a fluorescent lifetime using the picosecond stopwatch.
Imaging / Single
Molecule Instrument
In
conjunction with the laboratory of Elliot Elson, we have designed and assembled
two instruments for studying the fluorescent properties of samples. The first instrument
is for measuring the time-resolved fluorescent lifetimes of ensemble
cuvette-based samples to picosecond precision. The second instrument is for
measuring and imaging the fluorescence intensity of microscope samples down to
the single molecule level using either single or two-photon excitation. The
flexibility of both designs makes many types of experiments possible and allows
for future development of new measurement techniques. To learn more about these
instruments, please follow the links below.
We
have also developed some software utilities that streamline the process of
preparing FCS and Raw Photon data obtained from both the Zeiss Confocor2 and
the Correlator.com FLEX card, for subsequent fitting and analysis by
third-party software such as OriginLab’s Origin. Users can view files,
calculate means and standard deviations for multi-file sets, and perform
various calculations such as Photon Counting Histograms (PCH’s). To download
the software, please follow the FFS Utilities link below.
John
Jean measures a fluorescent lifetime using the picosecond stopwatch.
Imaging / Single
Molecule Instrument
Caroline
Clerte observes a molecule of RNA on the Single Molecule Instrument.
A
screen shot of one of the six panes of the FFS_Utilities Software.