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Saveez Saffarian PhD |
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Postdoctoral Researcher in Prof. Kirchhausen's Lab In the interface between the cytosol and the extra-cellular environment, plasma membrane is the initiation site for many complex multiprotein structures. These multiprotein structures which are in the range of 10-100nms control among others, signaling networks, endocytosis, excocyosis, enveloped virus egress and formation of immune synapse. Understanding the molecular mechanism of these events has been the focus of much research, however, most of our understanding has been derived from either fixed samples, purified components, or live cell imaging techniques with low spatial and temporal resolution. I have developed two methodologies to observe the multiprotein structures on the plasma membrane of a living cell with high spatial or temporal resolution. In the first approach called Differential Evanescence Nanometry (DINA) the axial separation of two groups of proteins are measured with 10 nanometer resolution in live cells. This new method has enabled the observation of localization of the components of a forming clathrin coated pit. In the second approach which is an extension of Fluorescence Intensity Distribution Analysis (FIDA) the molecular aggregation of different mobile proteins can be measured on the plasma membrane of a live cell. My current project is to understand the mechanism of clathrin coat formation in live cells using the above methodologies. Contact: Email: Saveez”at”hms.harvard.edu Phone: 617-335-7204 Address: Harvard Medical School/IDI 200 Longwood ave.
Last Updated 11/15/2007
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