murine Adenosine Deaminase (mADA)
19F Position and |
Chemical Shift Folded |
Chemical Shift Unfolded |
|
| Apo | 6-F-W117 | -45.15 | -46.15 |
| Apo | 6-F-W161 | -48.19 | -46.27 |
| Apo | 6-F-W264 | -47.59 | -46.27 |
| Apo | 6-F-W272 | -47.03 | -46.45 |
| DCF | 6-F-W117 | -44.82 | |
| DCF | 6-F-W161 | -47.73 | |
| DCF | 6-F-W264 | -47.55 | |
| DCF | 6-F-W272 | -46.85 | |
| PR | 6-F-W117 | -45.60 | |
| PR | 6-F-W161 | -47.98 | |
| PR | 6-F-W264 | -47.55 | |
| PR | 6-F-W272 | -46.85 |
Sample conditions: 20 mM Tris-HCl, pH 7.4, 2 mM DTT, 5% D2O, 20 degC.
Referenced to internal 0.2 mM 4-19F-Phe.
Biochemistry (2004) 43, 1432-1439
J. Mol. Biol. (2005) 345, 599-610
Dihidrofolate Reductase (DHFR)
19F Position and |
Chemical Shift
Folded |
Chemical Shift
Unfolded |
|
| Apo | 6-F-W22 | -45.6 | -46.1 |
| Apo | 6-F-W30 | -44.8 | -46.3 |
| Apo | 6-F-W47 | -49.2 | -46.3 |
| Apo | 6-F-W74 | -45.0 | -46.4 |
| Apo | 6-F-W133 | -46.9 | -46.5 |
| MTX | 6-F-W22 | -46.7 | |
| MTX | 6-F-W30 | -44.6 | |
| MTX | 6-F-W47 | -49.5 | |
| MTX | 6-F-W74 | -44.8 | |
| MTX | 6-F-W133 | -47.5 | |
| NADPH | 6-F-W22 | -44.0 | |
| NADPH | 6-F-W30 | -45.0 | |
| NADPH | 6-F-W47 | -49.3 | |
| NADPH | 6-F-W74 | -43.1 | |
| NADPH | 6-F-W133 | -46.9 | |
| MTX/NADPH | 6-F-W22 | -44.2 | |
| MTX/NADPH | 6-F-W30 | -45.0 | |
| MTX/NADPH | 6-F-W47 | -49.6 | |
| MTX/NADPH | 6-F-W74 | -42.9 | |
| MTX/NADPH | 6-F-W133 | -47.2 | |
| DHF | 6-F-W22 | -46.2 | |
| DHF | 6-F-W30 | -44.8 | |
| DHF | 6-F-W47 | -49.6 | |
| DHF | 6-F-W74 | -45.1 | |
| DHF | 6-F-W133 | -47.1 | |
| NADP | 6-F-W22 | -45.1 | |
| NADP | 6-F-W30 | -46.5 | |
| NADP | 6-F-W47 | -49.6 | |
| NADP | 6-F-W74 | -43.0 | |
| NADP | 6-F-W133 | -47.1 |
Sample
conditions: 50 mM phosphate pH 7.2, 0.1 M KCl, 0.1 mM EDTA, 1 nM DTT, 10%D2O
Referenced to external TFA at 0 ppm.
Intestinal Fatty-acid Bindng Protein (I-FABP)
19F Position and |
Chemical Shift Folded |
Chemical Shift Unfolded |
|
| Apo | 4-F-F2 | -37.52 | |
| Apo | 4-F-F17 | -37.29 | |
| Apo | 4-F-F47 | -38.84 | |
| Apo | 4-F-F55 | -40.72 | |
| Apo | 4-F-F62 | -47.16 | |
| Apo | 4-F-F68 | -44.80 | |
| Apo | 4-F-F93 | -38.26 | |
| Apo | 4-F-F128 | -41.38 | |
| Oleate | 4-F-F2 | -37.75 | |
| Oleate | 4-F-F17 | -35.65 | |
| Oleate | 4-F-F47 | -36.76 | |
| Oleate | 4-F-F55 | -39.24 | |
| Oleate | 4-F-F62 | -45.65 | |
| Oleate | 4-F-F68 | -40.16 | |
| Oleate | 4-F-F93 | -38.04 | |
| Oleate | 4-F-F128 | -39.43 |
Sample
conditions: 20 mM potassium phosphate pH 7.3, 0.25 mM EDTA, 8%D2O, 20degC.
Referenced to internal 6-19F-Tryptophan at -46.293 relative to TFA at 0 ppm.
Biochemistry (2005) 44, 2369-2377
19F Position and |
Chemical Shift
Folded |
Chemical Shift
Unfolded |
|
| Apo | 6-F-W36 | -46.7 | -46.37 |
| Apo | 6-F-W128 | -43.66 | -46.33 |
Sample conditions: 30 mM MOPS-HCl pH 7.0, 10%D2O, 20 degC.
Referenced to internal 0.3 mM 4-19F-Phe.
Proc. Natl. Acad. Sci. USA (2002) 99, 709-714