Wayne M. Barnes, Ph.D.

Associate Professor

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 212A McDonnell Sciences Building
Phone: 314-362-3351
Email: barneswm(at)wustl.edu


PCR methods; plant genetic engineering for insect or virus resistance

molecular model


  • Wu, E.Y., Walsh, A.R., Materne, E.C., Hiltner, E.P., Zielinski, B., Miller, B.R. 3rd, Mawby, L., Modeste, E., Parish, C.A. Barnes, W.M. and Kermekchiev, M.B.
    A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.
    Biochemistry 54:881-889 (2015). (Abstract)

  • Zhang, Z., Kermekchiev, M.B. and Barnes, W.M.
    Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq.
    J Mol Diagn. 12:152-161 (2010). (Abstract)

  • Kermekchiev, M.B., Kirilova, L.I., Vail, E.E. and Barnes, W.M.
    Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples.
    Nucleic Acids Res. 37:e40 (2009). (Abstract)

  • Ren, C.P., Chaudhuri, R.R., Fivian, A., Bailey, C.M., Antonio, M., Barnes, W.M. and Pallen, M.J.
    The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition.
    J Bacteriology 186:3547-3560 (2004). (Abstract)