Linda Pike's lab

Ronan, T., Macdonald-Obermann, J.L., Huelsmann, L., Bessman, N.J., Naegle, K.M. and Pike, L.J. (2016) Different EGF receptor agonists produce unique signatures for the recruitment of downstream signaling proteins. J Biol Chem 291:5528-5540 (Abstract)

Macdonald-Obermann, J.L. and Pike, L.J. (2014) Different EGF receptor ligands show distinct kinetics and biased or partial agonism for homodimer and heterodimer formation. J Biol Chem 289:26178-26188 (Abstract)

Macdonald-Obermann, J.L., Adak, S., Landgraf, R., Piwnica-Worms, D. and Pike, L.J. (2013) Dynamic analysis of the EGF receptor-ErbB2-ErbB3 Network by luciferase fragment complementation imaging. J Biol Chem 288:30773-30784 (Abstract)

Li, Y., Macdonald-Obermann, J., Westfall, C., Piwnica-Worms, D. and Pike, L.J. (2012) Quantitation of the effect of ErbB2 on EGF receptor binding and dimerization. J Biol Chem 287:31116-31125 (Abstract)

Pike, L.J. (2012) Negative co-operativity in the EGF receptor. Biochem Soc Trans 40:15-19 (PDF)

Macdonald-Obermann, J.L., Piwnica-Worms, D. and Pike, L.J. (2012) Mechanics of EGF receptor/ErbB2 kinase activation revealed by luciferase fragment complementation imaging. Proc Natl Acad Sci USA 109:137-142 (PDF)

Adak, S., Deandrade, D. and Pike, L.J. (2012) The tethering arm of the EGF receptor is required for negative cooperativity and signal transduction. J Biol Chem 286:1545-1555 (PDF)

Adak, S., Yang, K.S., Macdonald-Obermann, J. and Pike, L.J. (2011) The membrane proxima intracellular domain of the EGF receptor underlies negative cooperativity in ligand binding. J Biol Chem 286:45146-45155 (PDF)

Yang, K.S.,MacDonald-Obermann, J.L., Piwnica-Worms, D. and Pike, L.J. (2010) Asp-960/Glu-961 control the movement of the C-terminal tail of the EGF receptor to regulate asymmetric dimer formation. J Biol Chem 285:24014-24022 (PDF)

Yang, R.Y., Yang, K.S.,Pike, L.J. and Marshall, G.R. (2010) Targeting the dimerization of epidermal growth factor receptors with small-molecule inhibitors. Chem Biol Drug Des 76:1-9 (PDF)

Macdonald-Obermann, J.L. and Pike, L.J. (2009) The intercellular juxtamembrane domain of EGF receptor is responsible for the allosteric regulation of EGF binding. J Biol Chem 284:13570-13576 (PDF)

Macdonald-Obermann, J.L. and Pike, L.J. (2009) Palmitoylation of the EGF receptor impairs signal transduction and abolishes high-affinity ligand binding. Biochemistry 48:2505-2513 (PDF)

Yang, K.S., Ilagan, M.X., Piwnica-Worms, D. and Pike, L.J. (2009) Luciferase fragment complementation imaging of conformational changes in the EGF receptor. J Biol Chem 284:7474-7482 (PDF)

Pike, L.J. (2009) The challenge of lipid rafts. J Lipid Res 50:S323-S328 (PDF)

Eiblmaier, M., Meyer, L.A., Watson, M.A., Fracasso, P.M., Pike, L.J. and Anderson, C.J. (2008) Correlating EGFR expression with receptor-binding properties and internalization of 64Cu-DOTA-Cetuximab in 5 cervical cancer cell lines. J Nucl Med 49:1472-1479 (PDF)

Macdonald, J.L. and Pike L.J. (2008) Heterogeneity in EGF-binding affinities arises from negative cooperativity in an aggregating system. Proc Natl Acad Sci U S A. 105:112-117 (PDF)

Saffarian, S., Li, Y., Elson, E.L. and Pike, L.J. (2007) Oligomerization of the EGF receptor investigated by live cell fluorescence intensity distribution analysis. Biophys J 93:1021-1031(2007). (PDF)

Pike, L.J. (2006) Rafts defined. A report on the Keystone Symposium on Lipid Rafts and Cell Function. J Lipid Res 47:1597-1598. (PDF)

Macdonald, J., Li, Z., Su, W. and Pike, L.J. (2006) The membrane proximal disulfides of the EGF receptor extracellular domain are required for high affinity binding and signal transduction but do not play a role inlocalization of the receptor to lipid rafts. Biochim Biophy Acta 1763:870-878. (PDF)

Pike, L.J. (2005) Growth factor receptors, lipid rafts and caveolae: An evolving story. Biochim Biophy Acta 1746:260-273. (PDF)

Pike, L.J., Han, X. and Gross, R.W. (2005) EGFR are localized to lipid rafts that contain a balance of inner and outer leaflet lipids: A shotgun lipidomics study. J Biol Chem 280:26796-26804. (PDF)

Macdonald, J.L. and Pike, L.J. (2005) A Simplified Method for the Preparation of Detergent-Free Lipid Rafts. J. Lipid Research. 46:1061-1067. (PDF)

Pike, L.J. and Sadler, J.E. (2004) Proteomics, Genomics and the Future of Medical Education. Missouri Medicine. 101:496-499.

Pike, L.J. (2004) Lipid Rafts: Heterogeneity on the High Seas. Biochem. J. 378:281-292. (PDF)

Westover, E.J., Covery, D.F., Brockman, H.L., Brown, R.E. and Pike, L.J. (2003) "Cholesterol Depletion Results in Site-specific Increases in Epidermal Growth Factor Receptor Phosphorylation Due to Memberane Level Effects" J. Biol. Chem. 278:51125-51133. (PDF)

Pike, L.J. (2003) "Lipid Rafts: Bringing Order to Chaos" J. Lipid Res. 44:655-667. (PDF)

Pike, L.J. (2003) "Role of Lipid Domains in EGF Receptor Signaling" in Handbook of Cell Signaling. R.A. Bradshaw and E.A. Dennis, editors, Academic Press.

Pike, L.J. and Casey, L.A. (2002) Cholesterol Modulates EGF Receptor-Mediated Signaling by Altering Receptor Function and Trafficking. Biochem. 41:10315-10322. (PDF)

Sun, J., Nanjundan, M., Pike, L.J., Wiedmer, T. and Sims, P.J. (2002) Plasma membrane Phospholipid Scramblase 1 is Enriched in Lipid Rafts and Interacts with the Epidermal Growth Factor Receptor. Biochem. 41:6338-6345. (PDF)

Pike, L.J., Han, X., Chung, K.-N., and Gross, R.W. (2002) Lipid Rafts are Enriched in Arachidonic Acid and Plasmenylethanolamine and their Composition is Independent of Caveolin-1 Expression: A Quantitative Electrospray Ionization/Mass Spectormetric Analysis. Biochem. 41:2075-2088. (Abs)(PDF)

Loussouarn, G., Pike, L.J., Ashcroft, F.M., Makhina, E.N. and Nichols, C.G. (2001) Dynamic Sensitivity of KATP Channels to ATP. J. Biol. Chem. 276:29098-29103. (Abs)(PDF)

Shyng, S.-L., Barbieri, A., Gumusboga, A., Cukras, C., Pike, L., Davis, J.N., Stahl, P.D., and Nichols, C.G. (2000) Modulation of Nucleotide Sensitivity of KATP Channels by PI-4-P 5-Kinase. Proc. Natl. Acad. Sci. U.S.A., 97, 937-941. (Abs)(PDF)

Pike, L.J. and Miller, J.M. (1998) Cholesterol Depletion Delocalizes Phosphatidylinositol Bisphosphate and Inhibits Hormone-stimulated Phosphatidylinositol Turnover. J. Biol. Chem. 273: 22298-22304. (Abs)(PDF)

Liu, Y., Casey, L. and Pike, L.J. (1998) Compartmentalization of Phosphatidylinositol 4,5-Bisphosphate in Low-Density Membrane Domains in the Absence of Caveolin. Biochem. Biophys. Res. Commun. 245: 684-690. (Abs)(PDF)

Pandit, S.D., O'Hare, T., Donis-Keller, H. and Pike, L.J. (1997) Functional Characterization of an EGF Receptor/RET Chimera. J. Biol. Chem. 272: 2199-2206. (Abs)(PDF)

Pike, L.J. and Casey, L. (1996) Localization and Turnover of Phosphatidylinositol 4,5-biphosphate in Caveolin-Enriched Membrane Domains. J. Biol. Chem. 271: 26453-26456. (Abs)(PDF)

Hope, H.R. and Pike, L.J. (1996) Phosphoinositides and Phosphoinositide-utilizing Enzymes in Detergent-insoluble Lipid Domains. Mol. Biol. Cell 7: 843-851. (Abs)

Pandit, S.D., Donis-Keller, H., Iwanoto, T., Tomich, J.M. and Pike, L.J. (1996) The Multiple Endocrine Neoplasia Type 2B Point Mutation Alters Long-term Regulation and Enhances the Transforming Capacity of the Epidermal Growth FactorReceptor. J. Biol. Chem. 271: 5850-5858. (Abs)

Newberry, E.P. and Pike, L.J. (1995) Cell-Cycle-Dependent Modulation of EGF-Receptor-Mediator Signaling. Biochem. Biophys. Res. Commun. 208: 253-259. (Abs)

Hope, H.M.R. and Pike, L.J. (1994) Purification and Characterization of a Polyphosphoinositide Phosphatase from Rat Brain. J. Biol Chem. 269: 23648-23654. (Abs)

Schuh, S.M., Newberry, E.P., Dalton, M.A. and Pike, L.J. (1994) Mutation of proline-1003 to Glycine in the Epidermal Growth Factor (EGF) Receptor Enhances Responsiveness to EGF. Mol. Biol. Cell. 5: 739-746. (Abs)

Kuppuswamy, D., Dalton, M. and Pike, L.J. (1993) Serine 1002 is an In Vivo and In Vitro Site of Phosphorylation of the EGF Receptor. J. Biol. Chem. 268: 19134-19142. (Abs)

Pike, L.J. (1992) Phosphatidylinositol 4-Kinases and the Role of Polyphosphoinositides in Cellular Regulation. Endocrine Rev. 13: 692-706. (Abs)

Pike, L.J. (1992) Dimer Formation and Desensitization of the Epidermal Growth Factor Receptor J. Lab. Clin. Med. 120: 688-692.

Spizz, G. and Pike, L.J. (1992) Growth Factors Promote Uptake of Inositol in BC3H1 Cells. Biochem. Biophys. Res. Commun. 182: 1008-1015. (Abs)

Dadabay, C.Y., Patton, E., Cooper, J.A. and Pike, L.J. (1991) Lack of Correlation Between Changes in Polyphosphoinositide Levels and Actin/Gelsolin Complexes in A431 Cells Treated with Epidermal Growth Factor. J. Cell Biol 112: 1151-1156. (Abs)

Kuppuswamy, D. and Pike, L.J. (1991) Desensitization of the EGF Receptor Alters its Ability to Undergo EGF-Induced Dimerization. Cell Signal 3: 107-117. (Abs)

Walker, D.H. and Pike, L.J. (1990) Stimulation of Purified Phosphatidylinositol 4-Kinase by Cobra Venom Cardiotoxin. Biochim. Biophys. Acta 1055: 295-298. (Abs)

Dadabay, C.Y. and Pike, L.J. (1989) Purification and Characterization of a Cytosolic Transglutaminase from a Cultured Human Tumor Cell Line. Biochem. J. 264: 679-685. (Abs)

Cunningham, T., Kuppuswamy, D. and Pike, L.J. (1989) Treatment of A431 Cells with EGF Induces Desensitization of EGF-stimulated Phosphatidylinositol Turnover. J. Biol. Chem. 264: 15351-15356. (Abs)

Kuppuswamy, D. and Pike, L.J. (1989) Ligand Induced Desensitization of ¹²⁵I-EGF Internalization. J. Biol. Chem. 264: 3357-3363. (Abs)

Walker, D.W., Dougherty, N. and Pike, L.J. (1988) Purification and Characterization of a Phosphatidylinositol Kinase from A431 Cells. Biochem. 27: 6504-6511. (Abs)

Dadabay, C.Y. and Pike, L.J. (1987) Rapid Increase in the Transglutaminase Activity of A431 Cells Following Treatment with Epidermal Growth Factor. Biochem. 26: 6587-6591.

Walker, D.H. and Pike, L.J. (1987) A Phosphatidylinositol Kinase is Activated in Membranes Derived from Cells Treated with Epidermal Growth Factor. Proc. Natl. Acad. Sci. USA 84: 7513-7517. (Abs)(PDF)

Pike, L.J. and Eakes, A.T. (1987) Epidermal Growth Factor Stimulates the Production of Phosphatidylinositol-monophosphate and the Breakdown of Polyphosphoinositides in A431 Cells. J. Biol. Chem. 262: 1644-1651. (Abs)

Walker, D.H., Kuppuswamy, D., Visvanathan, A. and Pike, L.J. (1987) Substrate Specificity and Kinetic Mechanism of Human Placental Insulin Receptor/Kinase Biochem. 26: 1428-1433.

Pike, L.J. (1987) "Assay of Growth Factor-Stimulated Tyrosine Kinases Using Synthetic Peptide Substrates" in Methods in Enzymology 146: 353-362.

Stefanovic, D., Erikson, E., Pike, L.J. and Maller, J.L. (1986) Activation of a Ribosomal Protein S6 Protein Kinase in Xenopus Oocytes by Insulin and Insulin Receptor/Kinase. EMBO J. 5: 157-160.

Eide, B.L., Krebs, E.G., Ross, R., Pike, L.J. and Bowen-Pope, D.F. (1986) Tumor Promoter Enhances Mitogenesis by PDGF with Little Effect on PDGF Binding. J. Cell. Physiol. 126: 254-258.

Pike, L.J., Eakes, A.T. and Krebs, E.G. (1986) Characterization of Affinity-Purified Insulin Receptor/Kinase. Effects of Dithiothreitol on Receptor/Kinase Function. J. Biol. Chem. 261: 3782-3789.

Maller, J.L., Pike, L.J., Freidenberg, G.R., Cordera, R., Stith, B.J., Olefsky, J.M., and Krebs, E.G. (1986) Increased Phosphorylation of Ribosomal Protein S6 Following Microinjection of Insulin Receptor/Kinase into Xenopus Oocytes. Nature 320: 459-461.

Pike, L.J. and Krebs, E.G. (1986) "Protein Tyrosine Kinase Activity of Hormone and Growth Factor Receptors" in The Receptors, Academic Press (P. Michael Conn, Ed.) 93-134.

Benovic, J.L., Pike, L.J., Cerione, R.A., Staniszewski, C., Yoshimasa, T., Codina, J., Birnbaumer, L., Caron, M.G. and Lefkowitz, R.J. (1985) Phosphorylation of the Mammalian Beta-Adrenergic Receptor by Cyclic AMP-Dependent Protein Kinase: Regulation of the Rate of Receptor Phosphorylation and Dephosphorylation by Agonist Regulatory Protein. J. Biol. Chem. 260: 7094-7101.

Pike, L.J. Kuenzel, E.A, casnellie, J.E. and Krebs, E.G. (1984) A Comparison of the Insulin- and EGF-Stimulated Protein Kinases from Human Placenta J. Biol. Chem. 259: 9913-9921.

Cassie, D., Pike, L.J., Grant, G.A., Krebs, E.G. and Glaser, L. (1983) Interaction of Epidermal Growth Factor-Dependent Protein Kinase with Endogeneous Membrane Proteins and Soluble Peptide Substrate. J. Biol. Chem. 258: 2945-2950. (Abs)

Pike, L.J., Bowen-Pope, D.F., Ross, R. and Krebs, E.G. (1983) Characterization of Platelet-Derived Growth Factor-Stimulated Phosphorylation in Cell Membranes. J. Biol. Chem. 258: 9383-9390. (Abs)

Pike, L.J., Marquardt, J., Todaro, G.J., Gallis, B., Casnellie, J.E., Bornstein, P. and Krebs, E.G. (1982) Transforming Growth Factor and Epidermal Growth Factor Stimulate the Phosphorylation of a Synthetic, Tyrosine-Containing Peptide in a Similar Manner. J. Biol. Chem. 257: 14628-14631. (Abs)

Schaffhausen, B., Benjamin, T.L., Pike, L. Casnellie, J. and Krebs, E.G. (1982) Antibody to the Nonapeptide Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu is Specific for Polyoma Middle T Antigen and Inhibits In Vitro Kinase Activity. J. Biol. Chem. 257: 12467-12470. (Abs)

Pike, L.J., Gallis, B., Casnellie, J.E., Bornstein, P. and Krebs, E.G. (1982) Epidermal Growth Factor Stimulates the Phosphorylation of Synthetic Tyrosine-Containing Peptides by A431 Cell Membranes. Proc. Natl. Acad. Sci. USA 79: 1443-1447. (Abs)(PDF)

Casnellie, J.E., Harrison, M.L., Pike, L.J., Hellstrom, K.E. and Krebs, E.G. (1982) Phosphorylation of Synthetic Peptides by a Tyrosine Protein Kinase from the Particulate Fraction of a Lymphoma Cell Line. Proc. Natl. Acad. Sci. USA 79: 282-286. (Abs)(PDF)
Pike, L.J. and Lefkowitz, R.J. (1981) Correlation of Beta-Adrenergic Receptor-Stimulated [³H]GDP Release and Adenylate Cyclase Activation: Differences Between Frog and Turkey Reythrocyte Membranes J. Biol. Chem. 256: 2207-2212. (Abs)

Pike, L.J. and Lefkowitz, R.J. (1980) Use of Cell Fusion Techniques to Probe the Mechanism of Catecholamine-Induced Desensitization of Adenylate Cyclase in Frog Erythrocytes. Biochim. Biophys. Acta 632: 354-365.

Pike, L.J. and Lefkowitz, R.J. (1980) Parallel Activation and Desensitization of beta-Adrenergic Receptor-Coupled GTPase and Adenylate Cyclase in Frog and Turkey Erythrocytes. J. Biol. Chem. 255: 6860-6867. (Abs)

Pike, L.J., Limbird, L.E. and Lefkowitz, R.J. (1979) Beta-Adrenergic Receptors Determine Affinity but not Intrinsic Activity of Drugs for Stimulation of Adenylate Cyclase. Nature 280: 502-504.

Lefkowitz, R.J., Williams, L.T., Pike, L.J. and Wessels, M. (1978) Receptor Binding Studies of Beta-Adrenergic Receptors: New Insights into Activation and Desensitizaton of Adenylate Cyclase. Adv. Pharmacol. Therapeut. 1: 189-199.

Limbird, L.E., DeLean, A., Hickey, A.R., Pike, L.J. and Lefkowitz, R.J. (1978) Differential Effects of GTP on the Coupling of Beta-Adrenergic Receptors to Adenylate Cyclase from Frog and Turkey Erythrocytes: Application of New Graphical Methods for the Analysis of Receptor-Effector Coupling. Biochim. Biophys. Acta 586: 298-314.

Pike, L.J. and Lefkowitz, R.J. (1978) Multiple Effects of N,N' Dicyclohexyl Carbodiimide on the Beta-Adrenergic Receptor-Adenylate Cyclase System in Frog Erythrocytes. J. Cyclic Nucleotide Res. 4: 27-34.

Pike, L.J. and Lefkowitz, R.J. (1978) Agonist-Specific Alterations in Receptor Binding Affinity Associated with Solubilization of Turkey Erythrocyte Membrane Beta-Adrenergic Receptors. Mol. Pharmacol. 14: 370-375.