Imaging / Single Molecule Fluorescence Detection System Development

 

     The fluorescence imaging / single molecule detection system allows the study of fluorescent samples under the microscope. Samples are scanned and then areas of interest can be identified for further study. The changes in intensity with time of these areas can be analyzed and compared by a variety of methods. Dual APD detectors and easily configurable optics and light sources make possible Fluorescence Correlation Spectroscopy (FCS) measurements, Cross-Correlation measurements, FRET measurements, Anisotropy measurements, and Two-Photon Sample Excitation on a variety of samples ranging from cells to single molecules. Labview software is used to control the electronic components of the system from a user-friendly graphical interface.

 

            The instrument is composed of the optical system, the scanning system, the photon counting system, and the system software. To find out more about the components, follow the links below.

 

Optical System

Photon Counting System

Scanning System

Instrument Software

 

Last updated August 10, 2003