Timothy M. Lohman, Ph.D. – Department of Biochemistry and Molecular Biophysics

Brennecke Professor of Biophysics

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 2801 North Building
Phone: 314-362-4393
Email: lohman@wustl.edu

Research

Research in the Lohman Lab focuses on obtaining a molecular understanding of the mechanisms of protein-nucleic acid interactions involved in DNA metabolism, in particular, DNA motor proteins (helicases/translocases) and single stranded DNA binding proteins. Thermodynamic, kinetic, structural and single molecule approaches are used to probe these interactions at the molecular level.

Select Publications

Nicole Fazio, Kacey N Mersch, Linxuan Hao, Timothy M Lohman (2023). “E. coli RecBCD Nuclease Domain Regulates Helicase Activity but not Single Stranded DNA Translocase Activity” bioRxiv. [Preprint]. 2023 Oct 17:2023.10.13.561901. doi: 10.1101/2023.10.13.561901. (Abstract)

Evelyn Ploetz, Benjamin Ambrose, Anders Barth, Richard Börner, Felix Erichson, Achillefs N Kapanidis, Harold D Kim, Marcia Levitus, Timothy M Lohman, Abhishek Mazumder, David S Rueda, Fabio D Steffen, Thorben Cordes, Steven W Magennis & Eitan Lerner (2023). “A new twist on PIFE: photoisomerisation-related fluorescence enhancement” Methods Appl Fluoresc. 2023 Oct 12;12(1):012001. doi: 10.1088/2050-6120/acfb58. (Abstract)

Ankita Chadda, Alexander G Kozlov, Binh Nguyen, Timothy M Lohman, & Eric A Galburt (2024). “Mycobacterium tuberculosis Ku Stimulates Multi-round DNA Unwinding by UvrD1 Monomers” J Mol Biol. 2024 Jan 15;436(2):168367. doi: 10.1016/j.jmb.2023.168367. Epub 2023 Nov 14. (Abstract)

Evelyn Ploetz, Benjamin Ambrose, Anders Barth, Richard Börner, Felix Erichson, Achillefs N. Kapanidis, Harold D. Kim, Marcia Levitus, Timothy M. Lohman, Abhishek Mazumder, David S. Rueda, Fabio D. Steffen, Thorben Cordes, Steven W. Magennis, & Eitan Lerner (2023). “A new twist on PIFE: photoisomerisation-related fluorescence enhancement” ArXiv. 2023 Jul 10;arXiv:2302.12455v2. (Abstract)

Min Kyung Shinn, Sumit K. Chaturvedi, Alexander G. Kozlov, & Timothy M. Lohman (2023). “Allosteric effects of E. coli SSB and RecR proteins on RecO protein binding to DNA” Nucleic Acids Res. 2023 Mar 21;51(5):2284-2297. doi: 10.1093/nar/gkad084. (Abstract)

Alexander G. Kozlov, Xian Cheng, Hongshan Zhang, Min Kyung Shinn, Elizabeth Weiland, Binh Nguyen, Irina A. Shkel, Emily Zytkiewicz, Ilya J. Finkelstein, M. Thomas Record Jr., & Timothy M. Lohman (2022). “How Glutamate Promotes Liquid-liquid Phase Separation and DNA Binding Cooperativity of E. coli SSB Protein” J Mol Biol. 2022 Mar 26;434(9):167562. doi: 10.1016/j.jmb.2022.167562. Online ahead of print.

Linxuan Hao, Rui Zhang, & Timothy M. Lohman (2021). “Heterogeneity in E. coli RecBCD Helicase-DNA Binding and Base Pair Melting” J Mol Biol. 2021 Sep 3;433(18):167147. doi: 10.1016/j.jmb.2021.167147. Epub 2021 Jul 9. (Abstract)

Binh Nguyen, Min Kyung Shinn, Elizabeth Weiland, & Timothy M. Lohman (2021). “Regulation of E. coli Rep helicase activity by PriC” J Mol Biol. 2021 Jun 1;433(15):167072. doi: 10.1016/j.jmb.2021.167072. Online ahead of print. (Abstract)

Alexander G. Kozlov & Timothy M. Lohman (2021). “Probing E. coli SSB Protein-DNA topology by reversing DNA backbone polarity” Biophys J. 2021 Apr 20;120(8):1522-1533. doi: 10.1016/j.bpj.2021.02.025. Epub 2021 Feb 23. (Abstract)

Min Kyung Shinn, Alexander G. Kozlov, & Timothy M. Lohman (2021). “Allosteric effects of SSB C-terminal tail on assembly of E. coli RecOR proteins” Nucleic Acids Res. 2021 Feb 26;49(4):1987-2004. doi: 10.1093/nar/gkaa1291. (Abstract)

Katarzyna Dubiel, Camille Henry, Lisanne M. Spenkelink, Alexander G. Kozlov, Elizabeth A. Wood, Slobodan Jergic, Nicholas E. Dixon, Antoine M. van Oijen, Michael M. Cox, Timothy M. Lohman, Steven J. Sandler, & James L. Keck (2020). “Development of a Single-Stranded DNA-binding Protein Fluorescent Fusion Toolbox” Nucleic Acids Res. 2020 Jun 19;48(11):6053-6067. doi: 10.1093/nar/gkaa320. (Abstract)

Patrick McConnell, Marlene Mekel, Alex G. Kozlov, Olivia L. Mooren, Timothy M. Lohman, & John A. Cooper (2020). “Comparative Analysis of CPI-Motif Regulation of Biochemical Functions of Actin Capping Protein” Biochemistry. 2020 Mar 24;59(11):1202-1215. doi: 10.1021/acs.biochem.0c00092. Epub 2020 Mar 10. (Abstract)

Alexander G. Kozlov, Min Kyung Shinn, & Timothy M. Lohman (2019). “Regulation of Nearest-neighbor cooperative binding of E. coli SSB protein to DNA” Biophysical Journal. 28 October 2019. doi.org/10.1016/j.bpj.2019.09.047 (Abstract)

Weisz D.A., Johnson V.M., Niedzwiedzki D.M., Shinn M.K., Liu H., Klitzke C.F., Gross M.L., Blankenship R.E., Lohman T.M., & Pakrasi H.B. (2019). “A novel chlorophyll protein complex in the repair cycle of photosystem II.” Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21907-21913. doi: 10.1073/pnas.1909644116. Epub 2019 Oct 8. (Abstract)

Ordabayev Y.A., Nguyen B., Kozlov A.G., Jia H., & Lohman T.M. (2019). “UvrD helicase activation by MutL involves rotation of its 2B subdomain.” Proc Natl Acad Sci U S A. 2019 Jul 30. pii: 201905513. doi: 10.1073/pnas.1905513116. [Epub ahead of print] (Abstract)