Michael J. Greenberg, Ph.D. – Department of Biochemistry and Molecular Biophysics

Associate Professor

Biochemistry and Molecular Biophysics

Office: 253 McDonnell Sciences Building
Phone: 314-362-8670
Email: greenberg@wustl.edu

Research

The Greenberg Lab focuses on how cytoskeletal motors function in both health and disease. Currently, the lab is studying mutations that cause familial cardiomyopathies, the leading cause of sudden cardiac death in people under 30 years old. The lab uses an array of biochemical, biophysical, and cell biological techniques to decipher how these mutations affect heart contraction from the level of single molecules to the level of engineered tissues. Insights into the disease pathogenesis will guide efforts to develop novel therapies.

Select Publications

Michael J Greenberg (2024). “Super Relaxed or Super Stressed: Modeling Length-Dependent Activation in Cardiac Muscle” Biophys J.2024 Jul 26:S0006-3495(24)00492-2. doi: 10.1016/j.bpj.2024.07.033. (Abstract)

Ankit Garg, Silvia Jansen, Rui Zhang, Kory J Lavine & Michael J Greenberg (2024). “Dilated cardiomyopathy-associated skeletal muscle actin (ACTA1) mutation R256H disrupts actin structure and function and causes cardiomyocyte hypocontractility” bioRxiv. [Preprint]. 2024 Mar 12:2024.03.10.583979. doi: 10.1101/2024.03.10.583979. (Abstract)

Jasmine Cubuk, Lina Greenberg, Akiva E Greenberg, Ryan J Emenecker, Melissa D Stuchell-Brereton, Alex S Holehouse, Andrea Soranno & Michael J Greenberg (2024). “Structural dynamics of the intrinsically disordered linker region of cardiac troponin T” bioRxiv. [Preprint]. 2024 May 31:2024.05.30.596451. doi: 10.1101/2024.05.30.596451. (Abstract)

Ankit Garg, Silvia Jansen, Rui Zhang, Kory J Lavine, & Michael J Greenberg (2024). “Dilated cardiomyopathy-associated skeletal muscle actin (ACTA1) mutation R256H disrupts actin structure and function and causes cardiomyocyte hypocontractility” bioRxiv [Preprint]. 2024 Mar 12:2024.03.10.583979. doi: 10.1101/2024.03.10.583979 (Abstract)

Ankit Garg, Kory J Lavine, & Michael J Greenberg (2024). “Assessing Cardiac Contractility From Single Molecules to Whole Hearts” JACC Basic Transl Sci. 2023 Oct 11;9(3):414-439. doi: 10.1016/j.jacbts.2023.07.013. eCollection 2024 Mar. (Abstract)

Gretchen A Meyer, Jeremie L A Ferey, James A Sanford, Liam S Fitzgerald, Akiva E Greenberg, Kristoffer Svensson, Michael J Greenberg, & Simon Schenk (2024). “Insights into post-translational regulation of skeletal muscle contractile function by the acetyltransferases, p300 and CBP” bioRxiv [Preprint]. 2024 Feb 29:2024.02.27.582179. doi: 10.1101/2024.02.27.582179. (Abstract)

Shahnaz Ghahremani, Aditya Kanwal, Anthony Pettinato, Feria Ladha, Nicholas Legere, Ketan Thakar, Yanfen Zhu, Harianto Tjong, Andrea Wilderman, W Tom Stump, Lina Greenberg, Michael J Greenberg, Justin Cotney, Chia-Lin Wei, & J Travis Hinson (2024). “CRISPR Activation Reverses Haploinsufficiency and Functional Deficits Caused by TTN Truncation Variants” Circulation. 2024 Apr 16;149(16):1285-1297. doi: 10.1161/CIRCULATIONAHA.123.063972. Epub 2024 Jan 18. (Abstract)

Brent Scott & Michael J Greenberg (2023). “Multiscale biophysical models of cardiomyopathies reveal complexities challenging existing dogmas” Biophys J. 2023 Dec 19;122(24):4632-4634. doi: 10.1016/j.bpj.2023.11.014. Epub 2023 Nov 25. (Abstract)

Samantha K. Barrick & Michael J. Greenberg (2021). “Cardiac myosin contraction and mechanotransduction in health and disease” J Biol Chem. 2021 Nov;297(5):101297. doi: 10.1016/j.jbc.2021.101297. Epub 2021 Oct 9. (Abstract)

Nicole R. Wong, Jay Mohan, Benjamin J. Kopecky, Shuchi Guo, Lixia Du, Jamison Leid, Guoshuai Feng, Inessa Lokshina, Oleksandr Dmytrenko, Hannah Luehmann, Geetika Bajpai, Laura Ewald, Lauren Bell, Nikhil Patel, Andrea Bredemeyer, Carla J. Weinheimer, Jessica M. Nigro, Attila Kovacs, Sachio Morimoto, Peter O. Bayguinov, Max R. Fisher, W. Tom Stump, Michael Greenberg, James A. J. Fitzpatrick, Slava Epelman, Daniel Kreisel, Rajan Sah, Yongjian Liu, Hongzhen Hu, & Kory J. Lavine (2021). “Resident cardiac macrophages mediate adaptive myocardial remodeling” Immunity. 2021 Sep 14;54(9):2072-2088.e7. doi: 10.1016/j.immuni.2021.07.003. Epub 2021 Jul 27. (Abstract)

Maria Papadaki, Theerachat Kampaengsri, Samantha K. Barrick, Stuart G. Campbell, Dirk von Lewinski, Peter P. Rainer, Samantha P. Harris, Michael J. Greenberg, & Jonathan A. Kirk (2021). “Myofilament glycation in diabetes reduces contractility by inhibiting tropomyosin movement, is rescued by cMyBPC domains” J Mol Cell Cardiol. 2021 Sep 3;162:1-9. doi: 10.1016/j.yjmcc.2021.08.012. Online ahead of print. (Abstract)

Samantha K. Barrick, Lina Greenberg, & Michael J. Greenberg (2021). “A Troponin T Variant Linked with Pediatric Dilated Cardiomyopathy Reduces the Coupling of Thin Filament Activation to Myosin and Calcium Binding” Mol Biol Cell. 2021 Jun 23;mbcE21020082. doi: 10.1091/mbc.E21-02-0082. Online ahead of print. (Abstract)

Sarah R. Clippinger, Paige E. Cloonan, Wei Wang, Lina Greenberg, W. Tom Stump, Paweorn Angsutararux, Jeanne M. Nerbonne, & Michael J. Greenberg (2021). “Mechanical dysfunction of the sarcomere induced by a pathogenic mutation in troponin T drives cellular adaptation” J Gen Physiol. 2021 May 3;153(5):e202012787. doi: 10.1085/jgp.202012787. (Abstract)

Michael J. Greenberg & Jil C. Tardiff (2021). “Complexity in genetic cardiomyopathies and new approaches for mechanism-based precision medicine” J Gen Physiol. 2021 Mar 1;153(3):e202012662. doi: 10.1085/jgp.202012662. (Abstract)

Adam L. Bailey, Oleksandr Dmytrenko, Lina Greenberg, Andrea L. Bredemeyer, Pan Ma, Jing Liu, Vinay Penna, Lulu Lai, Emma S. Winkler, Sanja Sviben, Erin Brooks, Ajith P. Nair, Kent A. Heck, Aniket S. Rali, Leo Simpson, Mehrdad Saririan, Dan Hobohm, W. Tom Stump, James A. Fitzpatrick, Xuping Xie, Pei-Yong Shi, J. Travis Hinson, Weng-Tein Gi, Constanze Schmidt, Florian Leuschner, Chieh-Yu Lin, Michael S. Diamond, Michael J. Greenberg, & Kory J. Lavine (2021). “SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis” JACC Basic Transl Sci. 2021 Feb 26. doi: 10.1016/j.jacbts.2021.01.002. Online ahead of print. (Abstract)