Wayne M. Barnes, Ph.D.

Associate Professor

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 212A McDonnell Sciences Building
Phone: 314-362-3351
Email: barneswm@wustl.edu
 

Research

PCR methods; plant genetic engineering for insect or virus resistance

molecular model


Select Publications

Wu E.Y., Walsh A.R., Materne E.C., Hiltner E.P., Zielinski B., Miller B.R. 3rd, Mawby L., Modeste E., Parish C.A., Barnes W.M., & Kermekchiev M.B. (2015). “A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.” Biochemistry. 2015 Jan 27;54(3):881-9. doi: 10.1021/bi501198f. Epub 2015 Jan 9. (Abstract)

Zhang Z., Kermekchiev M.B., & Barnes W.M. (2010). “Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq.” J Mol Diagn. 2010 Mar;12(2):152-61. doi: 10.2353/jmoldx.2010.090070. Epub 2010 Jan 14. (Abstract)

Kermekchiev M.B., Kirilova L.I., Vail E.E., & Barnes W.M. (2009). “Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples.” Nucleic Acids Res. 2009 Apr;37(5):e40. doi: 10.1093/nar/gkn1055. Epub 2009 Feb 10. (Abstract)

Ren C.P., Chaudhuri R.R., Fivian A., Bailey C.M., Antonio M., Barnes W.M., & Pallen M.J. (2004). “The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition. J Bacteriol. 2004 Jun;186(11):3547-60. (Abstract)