Carl Frieden, Ph.D.

Carl Frieden

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 215 McDonnell Science Building
Phone: 314-362-3344


Protein folding, aggregation, intrinsically disordered proteins, fluorescence methods, ApoE lipoproteins and Alzheimer’s disease

Carl Frieden lab research

Select Publications

Mathivanan Chinnaraj, David A. Barrios, Carl Frieden, Tomasz Heyduk, Robert Flaumenhaft, & Nicola Pozzi. (2020). “Bioorthogonal Chemistry Enables Single-Molecule FRET Measurements of Catalytically Active Protein Disulfide Isomerase” Chembiochem. 2020 Aug 28. doi: 10.1002/cbic.202000537. Online ahead of print. (Abstract)

Eliza A. Ruben, Prafull S. Gandhi, Zhiwei Chen, Sarah K. Koester, Gregory T. DeKoster, Carl Frieden, & Enrico Di Cera. (2020). “19 F NMR reveals the conformational properties of free thrombin and its zymogen precursor prethrombin-2” J Biol Chem. 2020 Jun 12;295(24):8227-8235. doi: 10.1074/jbc.RA120.013419. Epub 2020 May 1. (Abstract)

John T. Petroff II, Ailing Tong, Lawrence Chen, Gregory T. DeKoster, Farha Khan, Jeff Abramson, Carl Frieden, & Wayland Cheng (2019). “Charge Reduction of Membrane Proteins in Native Mass Spectrometry Using Alkali Metal Acetate Salts” ChemRxiv. 2019-12-13. (Abstract)

Wang H., Eschweiler J., Cui W., Zhang H., Frieden C., Ruotolo B.T., & Gross M.L. (2019) “Native Mass Spectrometry, Ion Mobility, Electron-Capture Dissociation, and Modeling Provide Structural Information for Gas-Phase Apolipoprotein E Oligomers.” J Am Soc Mass Spectrom. 2019 Mar 18. doi: 10.1007/s13361-019-02148-z. [Epub ahead of print] (Abstract)

Frieden, C. (2019). “Protein oligomerization as a metabolic control mechanism: Application to apoE.” Protein Sci. 2019 Apr;28(4):837-842. doi: 10.1002/pro.3583. Epub 2019 Feb 18. (Abstract)

Melissa D. Stuchell-Brereton, Berevan Baban, Greg DeKoster, Carl Frieden, & Andrea Soranno. (2019). “Single-Molecule Conformational Analysis of Apolipoprotein E” Biophysical Journal. Volume 116, Issue 3, Supplement 1, 15 February 2019, Page 167a. (Abstract)

Klein R.D., Shu Q., Cusumano Z.T., Nagamatsu K., Gualberto N.C., Lynch A.J.L., Wu C., Wang W., Jain N., Pinkner J.S., Amarasinghe G.K., Hultgren S.J., Frieden C., & Chapman M.R. (2018). “Structure-Function Analysis of the Curli Accessory Protein CsgE Defines Surfaces Essential for Coordinating Amyloid Fiber Formation.” MBio. 2018 Jul 17;9(4). pii: e01349-18. doi: 10.1128/mBio.01349-18. (Abstract)

Wang H., Rempel D.L., Giblin D., Frieden C., & Gross M.L. (2017). “Peptide-Level Interactions between Proteins and Small-Molecule Drug Candidates by Two Hydrogen-Deuterium Exchange MS-Based Methods: The Example of Apolipoprotein E3.” Anal Chem. 2017 Oct 17;89(20):10687-10695. doi: 10.1021/acs.analchem.7b01121. Epub 2017 Sep 25. (Abstract)

Wang H., Shu Q., Rempel D.L., Frieden C., & Gross M.L. (2017). “Understanding Curli Amyloid-Protein Aggregation by Hydrogen-Deuterium Exchange and Mass Spectrometry.” Int J Mass Spectrom. 2017 Sep;420:16-23. doi: 10.1016/j.ijms.2016.10.006. Epub 2016 Oct 11. (Abstract)

Frieden C., Wang H., & Ho C.M.W. (2017). “A mechanism for lipid binding to apoE and the role of intrinsically disordered regions coupled to domain-domain interactions.” Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):6292-6297. doi: 10.1073/pnas.1705080114. Epub 2017 May 30. (Abstract)

Wang H., Shu Q., Frieden C., & Gross M.L. (2017). “Deamidation slows Curli Amyloid-Protein Aggregation.” Biochemistry. 2017 Jun 13;56(23):2865-2872. doi: 10.1021/acs.biochem.7b00241. Epub 2017 May 26. (Abstract)

Shu Q., Krezel A.M., Cusumano Z.T., Pinkner J.S., Klein R., Hultgren S.J., & Frieden C. (2016). “Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation.” Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7130-5. doi: 10.1073/pnas.1607222113. Epub 2016 Jun 13. (Abstract)

Mondal T., Wang H., DeKoster G.T., Baban B., Gross M.L., & Frieden C. (2016). “ApoE: In Vitro Studies of a Small Molecule Effector.” Biochemistry. 2016 May 10;55(18):2613-21. doi: 10.1021/acs.biochem.6b00324. Epub 2016 Apr 27. (Abstract)

Wang H., Shu Q., Rempel D.L., Frieden C., & Gross M.L. (2015). “Continuous and pulsed hydrogen-deuterium exchange and mass spectrometry characterize CsgE oligomerization.” Biochemistry. 2015 Oct 27;54(42):6475-81. doi: 10.1021/acs.biochem.5b00871. Epub 2015 Oct 14. (Abstract)

Frieden C. (2015). “ApoE: the role of conserved residues in defining function.” Protein Sci. 2015 Jan;24(1):138-44. doi: 10.1002/pro.2597. Epub 2014 Dec 9. (Abstract)