Eric Galburt, Ph.D.

Eric Galburt
Associate Professor

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 251 McDonnell Science Building
Phone: 314-362-5201
Email: egalburt@wustl.edu
 

Research

The Galburt lab studies the detailed molecular mechanisms of transcription initiation across the three domains of life. The lab specializes in using single-molecule and ensemble biophysical techniques including optical and magnetic trapping and fluorescence to monitor initiation in real-time. These techniques allow for the quantification of the rates and magnitudes of conformational transitions in RNA polymerase, its associated transcription initiation factors, and the promoter DNA template that ultimately underlie gene expression and its regulation.

Eric Galburt's research

Select Publications

Galburt E.A. (2018). “The calculation of transcript flux ratios reveals single regulatory mechanisms capable of activation and repression.” Proc Natl Acad Sci U S A. 2018 Nov 21. pii: 201809454. doi: 10.1073/pnas.1809454115. [Epub ahead of print] (Abstract)

Prusa J., Jensen D., Santiago-Collazo G., Pope S.S., Garner A.L., Miller J.J., Ruiz Manzano A., Galburt E.A., & Stallings C.L. (2018). “Domains within RbpA serve specific functional roles that regulate the expression of distinct mycobacterial gene subsets.” J Bacteriol. 2018 Apr 23. pii: JB.00690-17. doi: 10.1128/JB.00690-17. (Abstract)

Tomko E.J., Fishburn J., Hahn S., & Galburt E.A. (2017). “TFIIH generates a six-base-pair open complex during RNAP II transcription initiation and start-site scanning.” Nat Struct Mol Biol. 2017 Dec;24(12):1139-1145. doi: 10.1038/nsmb.3500. Epub 2017 Nov 6. (Abstract)

Galburt E.A. & Tomko E.J. (2017). “Conformational selection and induced fit as a useful framework for molecular motor mechanisms.” Biophys Chem. 2017 Apr;223:11-16. doi: 10.1016/j.bpc.2017.01.004. Epub 2017 Feb 3. (Abstract)

Garner A.L., Rammohan J., Huynh J.P., Onder L.M., Chen J., Bae B., Jensen D., Weiss L.A., Ruiz Manzano A., Darst S.A., Campbell E.A., Nickels B.E., Galburt E.A., & Stallings C.L. (2016). “Effects of Increasing the Affinity of CarD for RNA Polymerase on Mycobacterium tuberculosis Growth, rRNA Transcription, and Virulence.” J Bacteriol. 2017 Jan 30;199(4). pii: e00698-16. doi: 10.1128/JB.00698-16. Print 2017 Feb 15. (Abstract)

Galburt E.A. & Rammohan J. (2016). “A Kinetic Signature for Parallel Pathways: Conformational Selection and Induced Fit. Links and Disconnects between Observed Relaxation Rates and Fractional Equilibrium Flux under Pseudo-First-Order Conditions.” Biochemistry. 2016 Dec 20;55(50):7014-7022. Epub 2016 Dec 8. (Abstract)

Rammohan J., Ruiz Manzano A., Garner A.L., Prusa J. Stallings C.L., & Galburt E.A. (2016). “Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD.” Nucleic Acids Res. 2016 Sep 6;44(15):7304-13. doi: 10.1093/nar/gkw577. Epub 2016 Jun 24. (Abstract)

Fishburn J., Galburt E., & Hahn S. (2016). “Transcription Start Site Scanning and the Requirement for ATP during Transcription Initiation by RNA Polymerase II.” J Biol Chem. 2016 Jun 17;291(25):13040-7. doi: 10.1074/jbc.M116.724583. Epub 2016 Apr 17. (Abstract)

Lisica A., Engel C., Jahnel M., Roldán É., Galburt E.A., Cramer P., & Grill S.W. (2016). “Mechanisms of backtrack recovery by RNA polymerases I and II.” Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):2946-51. doi: 10.1073/pnas.1517011113. Epub 2016 Feb 29. (Abstract)

Fishburn J., Tomko E., Galburt E., & Hahn S. (2015). “Double-stranded DNA translocase activity of transcription factor TFIIH and the mechanism of RNA polymerase II open complex formation.” Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):3961-6. doi: 10.1073/pnas.1417709112. Epub 2015 Mar 16. (Abstract)

Rammohan J., Ruiz Manzano A., Garner A.L., Stallings C.L., & Galburt E.A. (2015). “CarD stabilizes mycobacterial open complexes via a two-tiered kinetic mechanism.” Nucleic Acids Res. 2015 Mar 31;43(6):3272-85. doi: 10.1093/nar/gkv078. Epub 2015 Feb 19. (Abstract)

Galburt E.A. (2014). “Magnetic Tweezers Force Spectroscopy” Handbook of Imaging in Biological Mechanics. Chapter 38. DOI: 10.1201/b17566-51. ISBN: 9781466588134. (Abstract)

Garner A.L., Weiss L., Manzano A.R., Galburt E.A., & Stallings C.L. (2014). “CarD integrates three functional modules to promote efficient transcription, antibiotic tolerance, and pathogenesis in mycobacteria.” Mol Microbiol. 2014 Aug;93(4):682-97. doi: 10.1111/mmi.12681. Epub 2014 Jul 16. (Abstract)

Galburt E.A., Tomko E.J., Stump W.T., & Ruiz Manzano A. (2014). “Force-dependent melting of supercoiled DNA at thermophilic temperatures.” Biophys Chem. 2014 Mar-Apr;187-188:23-8. doi: 10.1016/j.bpc.2014.01.001. Epub 2014 Jan 17. (Abstract)

Galburt E.A., Parrondo J.M., & Grill S.W. (2011). “RNA polymerase pushing.” Biophys Chem. 2011 Aug;157(1-3):43-7. doi: 10.1016/j.bpc.2011.04.009. Epub 2011 Apr 16. (Abstract)