Roberto Galletto, Ph.D.

Roberto Galletto
Associate Professor

Biochemistry and Molecular Biophysics

Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 2808 North Building
Phone: 314-362-4368
Email: galletto@wustl.edu
 

Research

Mechanistic studies of DNA motor proteins.

Galletto lab research

Select Publications

Dahan D., Tsirkas I., Dovrat D., Sparks M.A., Singh S.P., Galletto R., & Aharoni A. (2018). “Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures.” Nucleic Acids Res. 2018 Nov 5. doi: 10.1093/nar/gky1065. [Epub ahead of print] (Abstract)

Geronimo C.L., Singh S.P., Galletto R., & Zakian V.A. (2018). “The signature motif of the Saccharomyces cerevisiae Pif1 DNA helicase is essential in vivo for mitochondrial and nuclear functions and in vitro for ATPase activity.” Nucleic Acids Res. 2018 Sep 19;46(16):8357-8370. doi: 10.1093/nar/gky655. (Abstract)

Singh S.P., Kukshal V., De Bona P., Antony E., & Galletto R. (2018). “The mitochondrial single-stranded DNA binding protein from S. cerevisiae, Rim1, does not form stable homo-tetramers and binds DNA as a dimer of dimers.” Nucleic Acids Res. 2018 Jun 21. doi: 10.1093/nar/gky530. (Abstract)

Sokoloski J.E., Kozlov A.G., Galletto R., & Lohman T.M. (2016). “Chemo-mechanical pushing of proteins along single-stranded DNA.” Proc Natl Acad Sci U S A. 2016 May 31;113(22):6194-9. doi: 10.1073/pnas.1602878113. Epub 2016 May 16. (Abstract)

Koc K.N., Singh S.P., Stodola J.L., Burgers P.M., & Galletto R. (2016). “Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase δ.” Nucleic Acids Res. 2016 May 5;44(8):3811-9. doi: 10.1093/nar/gkw181. Epub 2016 Mar 21. (Abstract)

Singh, S.P., Koc, K.N., Stodola, J.L. and Galletto, R.
A Monomer of Pif1 Unwinds Double-Stranded DNA and It Is Regulated by the Nature of the Non-Translocating Strand at the 3′-End.
J Mol Biol 428:1053-1067 (2016). (Abstract)

Feldmann, E.A., De Bona, P. and Galletto, R.
The wrapping loop and Rap1 C-terminal (RCT) domain of yeast Rap1 modulate access to different DNA binding modes.
Journal of Biological Chemistry 290:11455-11466 (2015). (Abstract)

Koc, K.N., Stodola, J.L, Burgers, P.M. and Galletto, R.
Regulation of yeast DNA polymerase δ-mediated strand displacement synthesis by 5′-flaps.
Nucleic Acid Research 43:4179-4190. (2015). (Abstract)

Koc, K.N., Stodola, J.L., Burgers, P.M., and Galletto, R.
Regulation of yeast DNA polymerase δ-mediated strand displacement synthesis by 5′-flaps.
Nucleic Acid Research 43:4179-4190 (2015). (Abstract)

Feldmann, E.A., Koc, K.N. and Galletto, R.
Alternative arrangements of telomeric recognition sites regulate the binding mode of the DNA-binding domain of yeast Rap1.
Biophysical Chemistry 198:1-8 (2015). (Abstract)

Feldmann, E.A. and Galletto, R.
The DNA-binding domain of yeast Rap1 interacts with double-stranded DNA in multiple binding modes.
Biochemistry 33:7471-7483 (2014). (Abstract)

Nguyen B., Sokoloski J.E., Galletto R., Elson E.L., Wold M.S., & Lohman T.M. (2014). “Diffusion of human replication protein A along single-stranded DNA.” J Mol Biol. 2014 Sep 23;426(19):3246-3261. doi: 10.1016/j.jmb.2014.07.014. Epub 2014 Jul 22. (Abstract)

Galletto, R. and Tomko, E.
Translocation of Saccharomyces cerevisiae Pif1 helicase monomers on single-stranded DNA.
Nucleic Acids Res. 41:4613-4627 (2013). (Abstract)

Kozlov A.G., Galletto R., & Lohman T.M. (2012). “SSB-DNA binding monitored by fluorescence intensity and anisotropy.” Methods Mol Biol. 2012;922:55-83. doi: 10.1007/978-1-62703-032-8_4. (Abstract)

Barranco-Medina, S. and Galletto, R.
DNA binding induces dimerization of Saccharomyces cerevisiae Pif1.
Biochemistry 49:8445-8454 (2010). (Abstract)