Michael J. Greenberg, Ph.D.

Assistant Professor

Biochemistry and Molecular Biophysics

Lab Website
Publications (PubMed / NIH)

Office: 253 McDonnell Sciences Building
Phone: 314-362-8670
Email: greenberg@wustl.edu


The Greenberg Lab focuses on how cytoskeletal motors function in both health and disease. Currently, the lab is studying mutations that cause familial cardiomyopathies, the leading cause of sudden cardiac death in people under 30 years old. The lab uses an array of biochemical, biophysical, and cell biological techniques to decipher how these mutations affect heart contraction from the level of single molecules to the level of engineered tissues. Insights into the disease pathogenesis will guide efforts to develop novel therapies.

Select Publications

Jordan E Ezekian, Sarah R Clippinger, Jackie Garcia, Susan W Denfield, Aamir Jeewa, William Dreyer, Wenxin Zou, Yuxin Fan, Hugh D Allen, Jeffrey Kim, Michael Greenberg, & Andrew P Landstrom (2019). “The Mutation R94C InTNNT2-encoded Troponin T Predisposes to Restrictive Cardiomyopathy and Pediatric Sudden Death Through Impaired Thin Filament Relaxation Resulting in Myocardial Diastolic Dysfunction” American Heart Association, Inc. 11 Nov 2019, Circulation. 2019;140:A12139 (Abstract)

Clippinger S.R., Cloonan P.E., Greenberg L., Ernst M., Stump W.T., & Greenberg M.J. (2019). “Disrupted mechanobiology links the molecular and cellular phenotypes in familial dilated cardiomyopathy” Proc Natl Acad Sci U S A. 2019 Aug 19. pii: 201910962. doi: 10.1073/pnas.1910962116. [Epub ahead of print] (Abstract)

Barrick S.K., Clippinger S.R., Greenberg L., & Greenberg M.J. (2019). “Computational Tool to Study Perturbations in Muscle Regulation and Its Application to Heart Disease.” Biophys J. 2019 May 7. pii: S0006-3495(19)30379-0. doi: 10.1016/j.bpj.2019.05.002. [Epub ahead of print] (Abstract)

Porter J.R., Moeder K.E., Sibbald C.A., Zimmerman M.I., Hart K.M., Greenberg M.J., & Bowman G.R. (2019). “Cooperative Changes in Solvent Exposure Identify Cryptic Pockets, Switches, and Allosteric Coupling.” Biophys J. 2019 Mar 5;116(5):818-830. doi: 10.1016/j.bpj.2018.11.3144. Epub 2019 Jan 25. (Abstract)

Sarah R. Clippinger, Paige E. Cloonan, Lina Greenberg, William Stump, & Michael J. Greenberg. (2019). “A Familial Dilated Cardiomyopathy Mutation Decreases Myosin Generated Tension at the Molecular Level and Alters Mechanosensing at the Cellular Level” Biophysical Journal. Volume 116, Issue 3, Supplement 1, 15 February 2019, Page 30a. (Abstract)

Samantha K. Barrick & Michael J. Greenberg. (2019). “Molecular Mechanism of a Mutation Implicated in Pediatric-Onset Heart Disesase” Biophysical Journal. Volume 116, Issue 3, Supplement 1, 15 February 2019, Page 115a. (Abstract)

Woody M.S., Greenberg M.J., Barua B., Winkelmann D.A., Goldman Y.E., & Ostap E.M. (2018). “Positive cardiac inotrope omecamtiv mecarbil activates muscle despite suppressing the myosin working stroke.” Nat Commun. 2018 Sep 21;9(1):3838. doi: 10.1038/s41467-018-06193-2. (Abstract)

Greenberg M.J., Daily N.J., Wang A., Conway M.K., & Wakatsuki T. (2018). “Genetic and Tissue Engineering Approaches to Modeling the Mechanics of Human Heart Failure for Drug Discovery.” Front Cardiovasc Med. 2018 Sep 19;5:120. doi: 10.3389/fcvm.2018.00120. eCollection 2018. (Abstract)

Justin R Porter, Katelyn E Moeder, Carrie A Sibbald, Maxwell I Zimmerman, Kathryn M Hart, Michael J Greenberg, & Gregory R Bowman (2018). “Cooperative changes in solvent exposure identify cryptic pockets, conformational switches, and allosteric coupling” bioRxiv. September 18, 2018. doi.org/10.1101/323568. (Abstract)

Michael S. Woody, Michael J. Greenberg, Bipasha Barua, Donald A. Winkelmann, Yale E. Goldman, & E. Michael Ostap (2018). “Single Molecule, Optical Trapping Studies of Omecamtiv Mercarbil on Human Cardiac Myosin’s Force Production” Biophysical Journal, Volume 114, Issue 3, Supplement 1, 2 February 2018, Pages 317a-318a. doi.org/10.1016/j.bpj.2017.11.1787 (Abstract)

Sarah R. Clippinger, Lina Greenberg, & Michael J. Greenberg (2018). “Dissecting the Molecular Mechanism for Familial Cardiomyopathies” Biophysical Journal. Volume 114, Issue 3, Supplement 1, p139a–140a, 2 February 2018. doi: 10.1016/j.bpj.2017.11.787 (Abstract)

Paige E. Cloonan, Lina Greenberg, & Michael J.Greenberg (2018). “Mechanical and Structural Analysis of Cardiomyopathies at the Single Cell Level” Biophysical Journal, Volume 114, Issue 3, Supplement 1, 2 February 2018, Page 314a. doi.org/10.1016/j.bpj.2017.11.1771 (Abstract)

Greenberg M.J., Shuman H., & Ostap E.M. (2017). “Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers.” Methods Mol Biol. 2017;1486:483-509. doi: 10.1007/978-1-4939-6421-5_19. (Abstract)

Woody M.S., Lewis J.H., Greenberg M.J., Goldman Y.E., & Ostap E.M. (2016). “MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation.” Biophys J. 2016 Jul 26;111(2):273-282. doi: 10.1016/j.bpj.2016.06.019. (Abstract)

Greenberg M.J., Arpag G., Tuzel E., & Ostap E.M. (2016). “A Perspective on the Role of Myosins as Mechanosensors.” Biophys J. 2016 Jun 21;110(12):2568-2576. doi: 10.1016/j.bpj.2016.05.021. (Abstract)