Michael J. Greenberg, Ph.D.

Assistant Professor

Biochemistry and Molecular Biophysics

Lab Website
Publications (PubMed / NIH)

Office: 253 McDonnell Sciences Building
Phone: 314-362-8670
Email: greenberg@wustl.edu


The Greenberg Lab focuses on how cytoskeletal motors function in both health and disease. Currently, the lab is studying mutations that cause familial cardiomyopathies, the leading cause of sudden cardiac death in people under 30 years old. The lab uses an array of biochemical, biophysical, and cell biological techniques to decipher how these mutations affect heart contraction from the level of single molecules to the level of engineered tissues. Insights into the disease pathogenesis will guide efforts to develop novel therapies.

Select Publications

Woody M.S., Greenberg M.J., Barua B., Winkelmann D.A., Goldman Y.E., & Ostap E.M. (2018). “Positive cardiac inotrope omecamtiv mecarbil activates muscle despite suppressing the myosin working stroke.” Nat Commun. 2018 Sep 21;9(1):3838. doi: 10.1038/s41467-018-06193-2. (Abstract)

Sarah R. Clippinger, Lina Greenberg, & Michael J. Greenberg (2018). “Dissecting the Molecular Mechanism for Familial Cardiomyopathies” Biophysical Journal. Volume 114, Issue 3, Supplement 1, p139a–140a, 2 February 2018. doi: 10.1016/j.bpj.2017.11.787 (Abstract)

Greenberg M.J., Shuman H., & Ostap E.M. (2017). “Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers.” Methods Mol Biol. 2017;1486:483-509. doi: 10.1007/978-1-4939-6421-5_19. (Abstract)

Woody M.S., Lewis J.H., Greenberg M.J., Goldman Y.E., & Ostap E.M. (2016). “MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation.” Biophys J. 2016 Jul 26;111(2):273-282. doi: 10.1016/j.bpj.2016.06.019. (Abstract)

Greenberg M.J., Arpag G., Tuzel E., & Ostap E.M. (2016). “A Perspective on the Role of Myosins as Mechanosensors.” Biophys J. 2016 Jun 21;110(12):2568-2576. doi: 10.1016/j.bpj.2016.05.021. (Abstract)

Kee A.J., Yang L., Lucas C.A., Greenberg M.J., Martel N., Leong G.M., Hughes W.E., Cooney G.J., James D.E., Ostap E.M., Han W., Gunning P.W., & Hardeman E.C. (2015). “An actin filament population defined by the tropomyosin Tpm3.1 regulates glucose uptake.” Traffic. 2015 Jul;16(7):691-711. doi: 10.1111/tra.12282. Epub 2015 Apr 29. (Abstract)

Greenberg M.J., Lin T., Shuman H., & Ostap E.M. (2015). “Mechanochemical tuning of myosin-I by the N-terminal region.” Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3337-44. doi: 10.1073/pnas.1506633112. Epub 2015 Jun 8. (Abstract)

Greenberg M.J., Shuman H., & Ostap E.M. (2014). “Inherent force-dependent properties of β-cardiac myosin contribute to the force-velocity relationship of cardiac muscle.” Biophys J. 2014 Dec 16;107(12):L41-4. doi: 10.1016/j.bpj.2014.11.005. (Abstract)

Shuman H., Greenberg M.J., Zwolak A., Lin T., Sindelar C.V., Dominguez R., & Ostap E.M. (2014). “A vertebrate myosin-I structure reveals unique insights into myosin mechanochemical tuning.” Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2116-21. doi: 10.1073/pnas.1321022111. Epub 2014 Jan 27. (Abstract)

Greenberg M.J. & Ostap E.M. (2013). “Regulation and control of myosin-I by the motor and light chain-binding domains.” Trends Cell Biol. 2013 Feb;23(2):81-9. doi: 10.1016/j.tcb.2012.10.008. Epub 2012 Nov 29. (Abstract)

Greenberg M.J., Lin T., Goldman Y.E., Shuman H., & Ostap E.M. (2012). “Myosin IC generates power over a range of loads via a new tension-sensing mechanism.” Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):E2433-40. doi: 10.1073/pnas.1207811109. Epub 2012 Aug 20. (Abstract)

Lewis J.H., Greenberg M.J., Laakso J.M., Shuman H., & Ostap E.M. (2012). “Calcium regulation of myosin-I tension sensing.” Biophys J. 2012 Jun 20;102(12):2799-807. doi: 10.1016/j.bpj.2012.05.014. Epub 2012 Jun 19. (Abstract)

Chen, C., Greenberg, M.J., Laakso, J.M., Ostap, E.M., Goldman, Y.E and Shuman, H.
Kinetic schemes for post-synchronized single molecule dynamics.
Biophys J. 102:L23-L25 (2012). (Abstract)

Lin, T., Greenberg, M.J., Moore, J.R. and Ostap, E.M.
A hearing loss-associated myo1c mutation (R156W) decreases the myosin duty ratio and force sensitivity.
Biochemistry 50:1831-1838 (2011). (Abstract)

Greenberg, M.J., Kazmierczak, K., Szczesna-Cordary, D. and Moore JR.
Cardiomyopathy-linked myosin regulatory light chain mutations disrupt myosin strain-dependent biochemistry.
Proc Natl Acad Sci U S A 107:17403-17408 (2010). (Abstract)