Timothy M. Lohman, Ph.D.

Brennecke Professor of Biophysics

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 2801 North Building
Phone: 314-362-4393
Email: lohman@wustl.edu
 

Research


Research in the Lohman Lab focuses on obtaining a molecular understanding of the mechanisms of protein-nucleic acid interactions involved in DNA metabolism, in particular, DNA motor proteins (helicases/translocases) and single stranded DNA binding proteins. Thermodynamic, kinetic, structural and single molecule approaches are used to probe these interactions at the molecular level.

Select Publications

Katarzyna Dubiel, Camille Henry, Lisanne M. Spenkelink, Alexander G. Kozlov, Elizabeth A. Wood, Slobodan Jergic, Nicholas E. Dixon, Antoine M. van Oijen, Michael M. Cox, Timothy M. Lohman, Steven J. Sandler, & James L. Keck (2020). “Development of a Single-Stranded DNA-binding Protein Fluorescent Fusion Toolbox” Nucleic Acids Res. 2020 Jun 19;48(11):6053-6067. doi: 10.1093/nar/gkaa320. (Abstract)

Patrick McConnell, Marlene Mekel, Alex G. Kozlov, Olivia L. Mooren, Timothy M. Lohman, & John A. Cooper (2020). “Comparative analysis of CPI-motif regulation of biochemical functions of actin capping protein” bioRxiv. February 6th, 2020. doi.org/10.1101/2020.02.06.936211 (Abstract)

Alexander G. Kozlov, Min Kyung Shinn, & Timothy M. Lohman (2019). “Regulation of Nearest-neighbor cooperative binding of E. coli SSB protein to DNA” Biophysical Journal. 28 October 2019. doi.org/10.1016/j.bpj.2019.09.047 (Abstract)

Weisz D.A., Johnson V.M., Niedzwiedzki D.M., Shinn M.K., Liu H., Klitzke C.F., Gross M.L., Blankenship R.E., Lohman T.M., & Pakrasi H.B. (2019). “A novel chlorophyll protein complex in the repair cycle of photosystem II.” Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21907-21913. doi: 10.1073/pnas.1909644116. Epub 2019 Oct 8. (Abstract)

Ordabayev Y.A., Nguyen B., Kozlov A.G., Jia H., & Lohman T.M. (2019). “UvrD helicase activation by MutL involves rotation of its 2B subdomain.” Proc Natl Acad Sci U S A. 2019 Jul 30. pii: 201905513. doi: 10.1073/pnas.1905513116. [Epub ahead of print] (Abstract)

Shinn M.K., Kozlov A.G., Nguyen B., Bujalowski W.M., & Lohman T.M. (2019). “Are the intrinsically disordered linkers involved in SSB binding to accessory proteins?” Nucleic Acids Res. 2019 Jul 22. pii: gkz606. doi: 10.1093/nar/gkz606. [Epub ahead of print] (Abstract)

Nguyen B., Ciuba M.A., Kozlov A.G., Levitus M., & Lohman T.M. (2019). “Protein Environment and DNA Orientation Affect Protein-Induced Cy3 Fluorescence Enhancement.” Biophys J. 2019 Jun 7. pii: S0006-3495(19)30448-5. doi: 10.1016/j.bpj.2019.05.026. [Epub ahead of print] (Abstract)

Min Kyung Shinn, Alexander G. Kozlov, & Timothy M. Lohman. (2019). “Binding Specificity of E. coli SSB C-Terminal Tails to SIPs” Biophysical Journal. Volume 116, Issue 3, Supplement 1, 15 February 2019, Page 77a. (Abstract)

Makurath M.A., Whitley K.D., Nguyen B., Lohman T.M., & Chemla Y.R. (2019). “Regulation of Rep helicase unwinding by an auto-inhibitory subdomain.” Nucleic Acids Res. 2019 Mar 18;47(5):2523-2532. doi: 10.1093/nar/gkz023. (Abstract)

Dubiel K., Myers A.R., Kozlov A.G., Yang O., Zhang J., Ha T., Lohman T.M., & Keck J.L. (2018). “Structural Mechanisms of Cooperative DNA Binding by Bacterial Single-Stranded DNA-Binding Proteins.” J Mol Biol. 2018 Nov 22. pii: S0022-2836(18)31046-5. doi: 10.1016/j.jmb.2018.11.019. [Epub ahead of print] (Abstract)

Ordabayev Y.A., Nguyen B., Niedziela-Majka A., & Lohman T.M. (2018). “Regulation of UvrD Helicase Activity by MutL.” J Mol Biol. 2018 Aug 29. pii: S0022-2836(18)30591-6. doi: 10.1016/j.jmb.2018.08.022. (Abstract)

Johnson B., McConnell P., Kozlov A.G., Mekel M., Lohman T.M., Gross M.L., Amarasinghe G.K., & Cooper J.A. (2018). “Allosteric Coupling of CARMIL and V-1 Binding to Capping Protein Revealed by Hydrogen-Deuterium Exchange.” Cell Rep. 2018 May 29;23(9):2795-2804. doi: 10.1016/j.celrep.2018.04.096. (Abstract)

Lohman T.M. & Fazio N.T. (2018) “How Does a Helicase Unwind DNA? Insights from RecBCD Helicase.” Bioessays. 2018 Jun;40(6):e1800009. doi: 10.1002/bies.201800009. Epub 2018 Mar 30. (Abstract)

Antony E. & Lohman T.M. (2018) “Dynamics of E. coli single stranded DNA binding (SSB) protein-DNA complexes.” Semin Cell Dev Biol. 2018 Mar 30. pii: S1084-9521(17)30432-9. doi: 10.1016/j.semcdb.2018.03.017. [Epub ahead of print] (Abstract)

Min Kyung Shinn, Alexander G. Kozlov, & Timothy M. Lohman (2018). “Allosteric Effect of E. coli SSB C-Terminal Tails on RecOR Binding to DNA” Biophysical Journal, Volume 114, Issue 3, Supplement 1, 2 February 2018, Page 441a (Abstract)