Timothy M. Lohman, Ph.D.

Brennecke Professor of Biophysics

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 2801 North Building
Phone: 314-362-4393
Email: lohman@wustl.edu
 

Research


Research in the Lohman Lab focuses on obtaining a molecular understanding of the mechanisms of protein-nucleic acid interactions involved in DNA metabolism, in particular, DNA motor proteins (helicases/translocases) and single stranded DNA binding proteins. Thermodynamic, kinetic, structural and single molecule approaches are used to probe these interactions at the molecular level.

Select Publications

Lohman T.M. & Fazio N.T. (2018) “How Does a Helicase Unwind DNA? Insights from RecBCD Helicase.” Bioessays. 2018 Mar 30. doi: 10.1002/bies.201800009. [Epub ahead of print] (Abstract)

Antony E. & Lohman T.M. (2018) “Dynamics of E. coli single stranded DNA binding (SSB) protein-DNA complexes.” Semin Cell Dev Biol. 2018 Mar 30. pii: S1084-9521(17)30432-9. doi: 10.1016/j.semcdb.2018.03.017. [Epub ahead of print] (Abstract)

Min Kyung Shinn, Alexander G. Kozlov, & Timothy M. Lohman (2018). “Allosteric Effect of E. coli SSB C-Terminal Tails on RecOR Binding to DNA” Biophysical Journal, Volume 114, Issue 3, Supplement 1, Page 441a (Abstract)

Nguyen B., Ordabayev Y., Sokoloski J.E., Weiland E., & Lohman T.M. (2017). “Large domain movements upon UvrD dimerization and helicase activation.” Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):12178-12183. doi: 10.1073/pnas.1712882114. Epub 2017 Oct 30. (Abstract)

Tomko E.J. & Lohman T.M. (2017). “Modulation of Escherichia coli UvrD Single-Stranded DNA Translocation by DNA Base Composition.” Biophys J. 2017 Oct 3;113(7):1405-1415. doi: 10.1016/j.bpj.2017.08.023. (Abstract)

Kozlov A.G., Shinn M.K., Weiland E.A., & Lohman T.M. (2017). “Glutamate promotes SSB protein-protein Interactions via intrinsically disordered regions.” J Mol Biol. 2017 Sep 1;429(18):2790-2801. doi: 10.1016/j.jmb.2017.07.021. Epub 2017 Aug 3. (Abstract)

Simon M., Sokoloski J., Hao L., Weiland E., & Lohman T. (2016). “Processive DNA Unwinding by RecBCD Helicase in the Absence of Canonical Motor Translocation.” J Mol Biol. 2016 Jul 31;428(15):2997-3012. doi: 10.1016/j.jmb.2016.07.002. Epub 2016 Jul 14. (Abstract)

Waldman V.M., Weiland E., Kozlov A.G., & Lohman T.M. (2016). “Is a fully wrapped SSB-DNA complex essential for Escherichia coli survival?” Nucleic Acids Res. 2016 May 19;44(9):4317-29. doi: 10.1093/nar/gkw262. Epub 2016 Apr 15. (Abstract)

Sokoloski J.E., Kozlov A.G., Galletto R., & Lohman T.M. (2016). “Chemo-mechanical pushing of proteins along single-stranded DNA.” Proc Natl Acad Sci U S A. 2016 May 31;113(22):6194-9. doi: 10.1073/pnas.1602878113. Epub 2016 May 16. (Abstract)

Chowdhury F., Li I., Ngo T., Leslie B., Kim B., Sokoloski J., Weiland E., Wang X., Chemla Y., Lohman T., & Ha T. (2016). “Defining Single Molecular Forces Required for Notch Activation Using Nano Yoyo.” Nano Lett. 2016 Jun 8;16(6):3892-7. doi: 10.1021/acs.nanolett.6b01403. Epub 2016 May 12. (Abstract)

Suksombat S., Khafizov R., Kozlov A.G., Lohman T.M., & Chemla Y.R. (2015). “Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways.” Elife. 2015 Aug 25;4. doi: 10.7554/eLife.08193. (Abstract)

Petrova V., Chen S.H., Molzberger E.T., Tomko E., Chitteni-Pattu S., Jia H., Ordabayev Y., Lohman T.M., & Cox M.M. (2015). “Active displacement of RecA filaments by UvrD translocase activity.” Nucleic Acids Res. 2015 Apr 30;43(8):4133-49. doi: 10.1093/nar/gkv186. Epub 2015 Mar 30. (Abstract)

Comstock M.J., Whitley K.D., Jia H., Sokoloski J., Lohman T.M., Ha T., & Chemla Y.R. (2015). “Protein structure. Direct observation of structure-function relationship in a nucleic acid-processing enzyme.” Science. 2015 Apr 17;348(6232):352-4. doi: 10.1126/science.aaa0130. Epub 2015 Apr 16. (Abstract)

Kozlov A.G., Weiland E., Mittal A., Waldman V., Antony E., Fazio N., Pappu R.V., & Lohman T.M. (2015). “Intrinsically disordered C-terminal tails of E. coli single-stranded DNA binding protein regulate cooperative binding to single-stranded DNA.” J Mol Biol. 2015 Feb 27;427(4):763-74. doi: 10.1016/j.jmb.2014.12.020. Epub 2015 Jan 3. (Abstract)

Nguyen B., Sokoloski J.E., Galletto R., Elson E.L., Wold M.S., & Lohman T.M. (2014). “Diffusion of human replication protein A along single-stranded DNA.” J Mol Biol. 2014 Sep 23;426(19):3246-3261. doi: 10.1016/j.jmb.2014.07.014. Epub 2014 Jul 22. (Abstract)

Lee K.S., Marciel A.B., Kozlov A.G., Schroeder C.M., Lohman T.M., & Ha T. (2014). “Ultrafast redistribution of E. coli SSB along long single-stranded DNA via intersegment transfer.” J Mol Biol. 2014 Jun 26;426(13):2413-21. doi: 10.1016/j.jmb.2014.04.023. Epub 2014 May 2. (Abstract)

Antony E., Weiland E., Yuan Q., Manhart C.M., Nguyen B., Kozlov A.G., McHenry C.S., & Lohman T.M. (2013). “Multiple C-terminal tails within a single E. coli SSB homotetramer coordinate DNA replication and repair.” J Mol Biol. 2013 Nov 29;425(23):4802-19. doi: 10.1016/j.jmb.2013.08.021. Epub 2013 Sep 7. (Abstract)

Qiu Y., Antony E., Doganay S., Koh H.R., Lohman T.M., & Myong S. (2013). “Srs2 prevents Rad51 filament formation by repetitive motion on DNA.” Nat Commun. 2013;4:2281. doi: 10.1038/ncomms3281. (Abstract)

Lee K.S., Balci H., Jia H., Lohman T.M., & Ha T. (2013). “Direct imaging of single UvrD helicase dynamics on long single-stranded DNA.” Nat Commun. 2013;4:1878. doi: 10.1038/ncomms2882. (Abstract)

Antony E. & Lohman T.M. (2013). “Nonhexameric SF1 DNA Helicases/Translocases” Encyclopedia of Biological Chemistry, 2nd Edition, 2013, Pages 261-265. doi: 10.1016/B978-0-12-378630-2.00393-5. (Abstract)

Xie F., Wu C.G., Weiland E., & Lohman T.M. (2012). “Asymmetric regulation of bipolar single-stranded DNA translocation by the two motors within Escherichia coli RecBCD helicase.” J Biol Chem. 2013 Jan 11;288(2):1055-64. doi: 10.1074/jbc.M112.423384. Epub 2012 Nov 27. (Abstract)

Kozlov A.G., Galletto R., & Lohman T.M. (2012). “SSB-DNA binding monitored by fluorescence intensity and anisotropy.” Methods Mol Biol. 2012;922:55-83. doi: 10.1007/978-1-62703-032-8_4. (Abstract)

Wu C.G., Xie F., & Lohman T.M. (2012). “The primary and secondary translocase activities within E. coli RecBC helicase are tightly coupled to ATP hydrolysis by the RecB motor.” J Mol Biol. 2012 Oct 26;423(3):303-14. doi: 10.1016/j.jmb.2012.07.009. Epub 2012 Jul 20. (Abstract)

Kozlov A.G. & Lohman T.M. (2012). “SSB binding to ssDNA using isothermal titration calorimetry.” Methods Mol Biol. 2012;922:37-54. doi: 10.1007/978-1-62703-032-8_3. (Abstract)

Antony E., Weiland E.A., Korolev S., & Lohman T.M. (2012). “Plasmodium falciparum SSB tetramer wraps single-stranded DNA with similar topology but opposite polarity to E. coli SSB.” J Mol Biol. 2012 Jul 20;420(4-5):269-83. doi: 10.1016/j.jmb.2012.04.021. Epub 2012 Apr 27. (Abstract)