Timothy M. Lohman, Ph.D.

Brennecke Professor of Biophysics

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 2801 North Building
Phone: 314-362-4393
Email: lohman@wustl.edu
 

Research


Research in the Lohman Lab focuses on obtaining a molecular understanding of the mechanisms of protein-nucleic acid interactions involved in DNA metabolism, in particular, DNA motor proteins (helicases/translocases) and single stranded DNA binding proteins. Thermodynamic, kinetic, structural and single molecule approaches are used to probe these interactions at the molecular level.

Select Publications

Ordabayev Y.A., Nguyen B., Niedziela-Majka A., & Lohman T.M. 2018. “Regulation of UvrD Helicase Activity by MutL.” J Mol Biol. 2018 Aug 29. pii: S0022-2836(18)30591-6. doi: 10.1016/j.jmb.2018.08.022. (Abstract)

Johnson B., McConnell P., Kozlov A.G., Mekel M., Lohman T.M., Gross M.L., Amarasinghe G.K., & Cooper J.A. (2018). “Allosteric Coupling of CARMIL and V-1 Binding to Capping Protein Revealed by Hydrogen-Deuterium Exchange.” Cell Rep. 2018 May 29;23(9):2795-2804. doi: 10.1016/j.celrep.2018.04.096. (Abstract)

Lohman T.M. & Fazio N.T. (2018) “How Does a Helicase Unwind DNA? Insights from RecBCD Helicase.” Bioessays. 2018 Mar 30. doi: 10.1002/bies.201800009. [Epub ahead of print] (Abstract)

Antony E. & Lohman T.M. (2018) “Dynamics of E. coli single stranded DNA binding (SSB) protein-DNA complexes.” Semin Cell Dev Biol. 2018 Mar 30. pii: S1084-9521(17)30432-9. doi: 10.1016/j.semcdb.2018.03.017. [Epub ahead of print] (Abstract)

Min Kyung Shinn, Alexander G. Kozlov, & Timothy M. Lohman (2018). “Allosteric Effect of E. coli SSB C-Terminal Tails on RecOR Binding to DNA” Biophysical Journal, Volume 114, Issue 3, Supplement 1, Page 441a (Abstract)

Nguyen B., Ordabayev Y., Sokoloski J.E., Weiland E., & Lohman T.M. (2017). “Large domain movements upon UvrD dimerization and helicase activation.” Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):12178-12183. doi: 10.1073/pnas.1712882114. Epub 2017 Oct 30. (Abstract)

Tomko E.J. & Lohman T.M. (2017). “Modulation of Escherichia coli UvrD Single-Stranded DNA Translocation by DNA Base Composition.” Biophys J. 2017 Oct 3;113(7):1405-1415. doi: 10.1016/j.bpj.2017.08.023. (Abstract)

Kozlov A.G., Shinn M.K., Weiland E.A., & Lohman T.M. (2017). “Glutamate promotes SSB protein-protein Interactions via intrinsically disordered regions.” J Mol Biol. 2017 Sep 1;429(18):2790-2801. doi: 10.1016/j.jmb.2017.07.021. Epub 2017 Aug 3. (Abstract)

Simon M., Sokoloski J., Hao L., Weiland E., & Lohman T. (2016). “Processive DNA Unwinding by RecBCD Helicase in the Absence of Canonical Motor Translocation.” J Mol Biol. 2016 Jul 31;428(15):2997-3012. doi: 10.1016/j.jmb.2016.07.002. Epub 2016 Jul 14. (Abstract)

Waldman V.M., Weiland E., Kozlov A.G., & Lohman T.M. (2016). “Is a fully wrapped SSB-DNA complex essential for Escherichia coli survival?” Nucleic Acids Res. 2016 May 19;44(9):4317-29. doi: 10.1093/nar/gkw262. Epub 2016 Apr 15. (Abstract)

Sokoloski J.E., Kozlov A.G., Galletto R., & Lohman T.M. (2016). “Chemo-mechanical pushing of proteins along single-stranded DNA.” Proc Natl Acad Sci U S A. 2016 May 31;113(22):6194-9. doi: 10.1073/pnas.1602878113. Epub 2016 May 16. (Abstract)

Chowdhury F., Li I., Ngo T., Leslie B., Kim B., Sokoloski J., Weiland E., Wang X., Chemla Y., Lohman T., & Ha T. (2016). “Defining Single Molecular Forces Required for Notch Activation Using Nano Yoyo.” Nano Lett. 2016 Jun 8;16(6):3892-7. doi: 10.1021/acs.nanolett.6b01403. Epub 2016 May 12. (Abstract)

Suksombat S., Khafizov R., Kozlov A.G., Lohman T.M., & Chemla Y.R. (2015). “Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways.” Elife. 2015 Aug 25;4. doi: 10.7554/eLife.08193. (Abstract)

Petrova V., Chen S.H., Molzberger E.T., Tomko E., Chitteni-Pattu S., Jia H., Ordabayev Y., Lohman T.M., & Cox M.M. (2015). “Active displacement of RecA filaments by UvrD translocase activity.” Nucleic Acids Res. 2015 Apr 30;43(8):4133-49. doi: 10.1093/nar/gkv186. Epub 2015 Mar 30. (Abstract)

Comstock M.J., Whitley K.D., Jia H., Sokoloski J., Lohman T.M., Ha T., & Chemla Y.R. (2015). “Protein structure. Direct observation of structure-function relationship in a nucleic acid-processing enzyme.” Science. 2015 Apr 17;348(6232):352-4. doi: 10.1126/science.aaa0130. Epub 2015 Apr 16. (Abstract)