This course is designed for graduate students and upper level undergraduate students who have a basic knowledge in molecular biology and nucleic acid biochemistry from appropriate undergraduate classes. Experience in organic chemistry is also very useful. Formal lectures in all areas of nucleic acids structure and function are complemented with discussions of current literature. The purpose of these discussion sessions is to gain a critical understanding of approaches and methodologies used to address basic problems in molecular biology.
Our general course philosophy is to help the student become a graduate student, which means learning how to do science. We want to avoid simply covering a textbook’s worth of facts in cell biology; most of the students have had cell biology material in previous courses. The lecture series is designed to emphasize how experiments are designed and interpreted, coupled with an understanding of the current status of research. The overall objective is to allow an understanding of the strategies and principles used to investigate fundamental concepts in cell biology, which we hope will be a valuable beginning to the independent research you will do as a graduate student.
Molecular motors in the cell harness chemical energy to generate mechanical work in a host of processes including cell motility, DNA replication and repair, cell division, transcriptional regulation, and intracellular transport. The purpose of this course is to discuss recent advances in the field of molecular motors. Special emphasis will be placed on understanding and critically evaluating single molecule studies. The course will consist of both journal club presentations and small group discussions.
This course will discuss the theoretical principles, logic and approaches used to study these processes from a quantitative perspective. Topics will include thermodynamics, multiple binding equilibria, analysis of binding isotherms, Wyman linkage relationships, cooperativity, allostery, macromolecular assembly, enzyme catalysis and mechanism, steady-state and pre-steady state kinetics, as well as single molecule approaches. Some techniques used to study binding processes will also be discussed. The student will also learn to use computer simulation and non-linear least squares methods to aid in the analysis of both equilibrium binding and kinetic studies.
This course is designed primarily for medical students and will cover fundamental aspects of biochemistry and cell biology. The course begins with a treatment of protein structure and folding and includes coverage of protein folding diseases. The principles of enzyme kinetics and regulation are then discussed to round out the protein chemistry section of the course.
Biology 5357 explores the physicochemical basis for the structural integrity, thermodynamic properties and biological functions of proteins, nucleic acids and lipid membranes
The purpose of this journal club is to discuss recent research studies of the mechanism and function of macromolecular machines.
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