Category: Holehouse Publications

Structural dynamics of the intrinsically disordered linker region of cardiac troponin T

Jasmine Cubuk, Lina Greenberg, Akiva E Greenberg, Ryan J Emenecker, Melissa D Stuchell-Brereton, Alex S Holehouse, Andrea Soranno & Michael J Greenberg (2024). “Structural dynamics of the intrinsically disordered linker region of cardiac troponin T” bioRxiv. [Preprint]. 2024 May 31:2024.05.30.596451. doi: 10.1101/2024.05.30.596451. (Abstract)

The disordered N-terminal tail of SARS-CoV-2 Nucleocapsid protein forms a dynamic complex with RNA

Jasmine Cubuk, Jhullian J Alston, J Jeremías Incicco, Alex S Holehouse, Kathleen B Hall, Melissa D Stuchell-Brereton & Andrea Soranno (2024). “The disordered N-terminal tail of SARS-CoV-2 Nucleocapsid protein forms a dynamic complex with RNA” Nucleic Acids Res. 2024 Mar 21;52(5):2609-2624. doi: 10.1093/nar/gkad1215. (Abstract)

Conformational buffering underlies functional selection in intrinsically disordered protein regions

Nicolás S. González-Foutel, Juliana Glavina, Wade M. Borcherds, Matías Safranchik, Susana Barrera-Vilarmau, Amin Sagar, Alejandro Estaña, Amelie Barozet, Nicolás A. Garrone, Gregorio Fernandez-Ballester, Clara Blanes-Mira, Ignacio E. Sánchez, Gonzalo de Prat-Gay, Juan Cortés, Pau Bernadó, Rohit V. Pappu, Alex S. Holehouse, Gary W. Daughdrill, & Lucía B. Chemes (2022). “Conformational buffering underlies functional selection in intrinsically disordered protein regions” Nat Struct Mol Biol. 2022 Aug;29(8):781-790. doi: 10.1038/s41594-022-00811-w. Epub 2022 Aug 10. (Abstract)

Metapredict: a fast, accurate, and easy-to-use predictor of consensus disorder and structure

Ryan J. Emenecker, Daniel Griffith, & Alex S. Holehouse (2021). “Metapredict: a fast, accurate, and easy-to-use predictor of consensus disorder and structure” Biophys J. 2021 Sep 2;S0006-3495(21)00725-6. doi: 10.1016/j.bpj.2021.08.039. Online ahead of print. (Abstract)

A prion-like protein regulator of seed germination undergoes hydration-dependent phase separation

Yanniv Dorone, Steven Boeynaems, Eduardo Flores, Benjamin Jin, Shannon Hateley, Flavia Bossi, Elena Lazarus, Janice G. Pennington, Emiel Michiels, Mathias De Decker, Katlijn Vints, Pieter Baatsen, George W. Bassel, Marisa S. Otegui, Alex S. Holehouse, Moises Exposito-Alonso, Shahar Sukenik, Aaron D. Gitler, Seung Y. Rhee (2021). “A prion-like protein regulator of seed germination undergoes hydration-dependent phase separation” Cell. 2021 Aug 5;184(16):4284-4298.e27. doi: 10.1016/j.cell.2021.06.009. Epub 2021 Jul 6. (Abstract)

Sequence determinants of in cell condensate morphology, dynamics, and oligomerization as measured by number and brightness analysis

Ryan J. Emenecker, Alex S. Holehouse, & Lucia C. Strader (2021). “Sequence determinants of in cell condensate morphology, dynamics, and oligomerization as measured by number and brightness analysis” Cell Commun Signal. 2021 Jun 5;19(1):65. doi: 10.1186/s12964-021-00744-9. (Abstract)