Eric Galburt, Ph.D.

Eric Galburt
Assistant Professor

Biochemistry and Molecular Biophysics

Lab Website
Graduate Programs (DBBS)
Publications (PubMed / NIH)

Office: 251 McDonnell Science Building
Phone: 314-362-5201
Email: egalburt(at)wustl.edu
 

Research

The Galburt lab studies the detailed molecular mechanisms of transcription initiation across the three domains of life. The lab specializes in using single-molecule and ensemble biophysical techniques including optical and magnetic trapping and fluorescence to monitor initiation in real-time. These techniques allow for the quantification of the rates and magnitudes of conformational transitions in RNA polymerase, its associated transcription initiation factors, and the promoter DNA template that ultimately underlie gene expression and its regulation.

Eric Galburt's research

Publications

  • Galburt E.A. and Tomko E.J.
    Conformational selection and induced fit as a useful framework for molecular motor mechanisms.
    Biophys Chem. 223:11-16 (2017) (Abstract)

  • Galburt E.A. and Rammohan J.
    A Kinetic Signature for Parallel Pathways: Conformational Selection and Induced Fit. Links and Disconnects between Observed Relaxation Rates and Fractional Equilibrium Flux under Pseudo-First-Order Conditions.
    Biochemistry. 7014-7022. (2016) (Abstract)

  • Galburt E.A. and Rammohan J.
    A Kinetic Signature for Parallel Pathways: Conformational Selection and Induced Fit. Links and Disconnects between Observed Relaxation Rates and Fractional Equilibrium Flux under Pseudo-First-Order Conditions.
    Biochemistry. 20;55(50):7014-7022 (2016) (Abstract)

  • Garner A.L., Rammohan J., Huynh J.P., Onder L.M., Chen J., Bae B., Jensen D., Weiss L.A., Ruiz Manzano A., Darst S.A., Campbell E.A., Nickels B.E., Galburt E.A., and Stallings C.L.
    Effects of Increasing the Affinity of CarD for RNA Polymerase on Mycobacterium tuberculosis Growth, rRNA Transcription, and Virulence.
    J Bacteriol. (2016) (Abstract)

  • Rammohan, J., Ruiz Manzano, A., Garner, A.L., Prusa, J. Stallings, C.L. and Galburt, E.A.
    Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD.
    Nucleic Acids Research
    Volume 44, Issue 15, Pages 7304-7313 (2016) (Abstract)

  • Rammohan, J., Ruiz Manzano, A., Garner, A.L., Prusa, J., Stallings, C.L. and Galburt, E.A. Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD. Nucleic Acid Res. (E-pub ahead of print.) (2016). [Abstract]
  • Lisica, A., Engel, C., Jahnel, M., Roldan, E., Galburt, E.A., Cramer, P. and Grill, S.W. Mechanisms of backtrack recovery by RNA polymerases I and II. PNAS 113:2946-2951 (2016). [Abstract]
  • Fishburn, J., Tomko, E. Galburt, E.A. and Hahn, S. Double-stranded DNA translocase activity of transcription factor TFIIH and the mechanism of RNA polymerase II open complex formation. PNAS 112:3961-3966 (2015). [Abstract]
  • Rammohan, J., Ruiz Manzano, A., Garner, A.L., Stallings, C.L. and Galburt, E.A. CarD stabilizes mycobacterial open complexes via a two-tiered kinetic mechanism. Nucleic Acids Research 43:3272-3285 (2015). [Abstract]
  • Garner,A.L., Weiss, L.A., Ruiz Manzano, A., Galburt, E.A. and Stallings, C.L. CarD integrates three functional modules to promote efficient transcription, antibiotic tolerance, and pathogenesis in Mycobacteria. Mol Microbiol 93:682-697 (2014). [Abstract]
  • Galburt, E.A., Tomko, E.J., Stump, W.T. and Ruiz Manzano, A. Force-dependent melting of supercoiled DNA at thermophilic temperatures. Biophys Chem. 187-188:23-28 (2014). [Abstract]
  • Galburt, E.A. Magnetic tweezers force spectroscopy. Handbook of Imaging in Biological Mechanics Chapter 38, 481-490. (2014). [Abstract]
  • Galburt, E.A., Grill, S.W. and Bustamante, C. Single molecule transcription elongation. Methods 48:323-332 (2009). [Abstract]

  • Depken, M, Galburt, E.A. and Grill, S.W. The origin of short transcriptional pauses. Biophys J 96:2189-2193 (2009). [Abstract]

  • Galburt. E.A., Grill, S.W., Wiedmann, A., Lubkowska, L., Choy, J., Nogales, E., Kashlev, M., and Bustamante, C. Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner. Nature, 446:820-823 (2007). [Abstract]