Dr. Barnes’ Publications
Wayne M. Barnes, Zhian Zhang, & Milko B. Kermekchiev (2021). “A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement” Front. Bioeng. Biotechnol., 14 January 2021, doi: 10.3389/fbioe.2020.553474 (Abstract)
Wu E.Y., Walsh A.R., Materne E.C., Hiltner E.P., Zielinski B., Miller B.R. 3rd, Mawby L., Modeste E., Parish C.A., Barnes W.M., & Kermekchiev M.B. (2015). “A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.” Biochemistry. 2015 Jan 27;54(3):881-9. doi: 10.1021/bi501198f. Epub 2015 Jan 9. (Abstract)
Zhang Z., Kermekchiev M.B., & Barnes W.M. (2010). “Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq.” J Mol Diagn. 2010 Mar;12(2):152-61. doi: 10.2353/jmoldx.2010.090070. Epub 2010 Jan 14. (Abstract)
Kermekchiev M.B., Kirilova L.I., Vail E.E., & Barnes W.M. (2009). “Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples.” Nucleic Acids Res. 2009 Apr;37(5):e40. doi: 10.1093/nar/gkn1055. Epub 2009 Feb 10. (Abstract)
Ren C.P., Chaudhuri R.R., Fivian A., Bailey C.M., Antonio M., Barnes W.M., & Pallen M.J. (2004). “The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition. J Bacteriol. 2004 Jun;186(11):3547-60. (Abstract)